Baele Margo, Vancanneyt Marc, Devriese Luc A, Lefebvre Karen, Swings Jean, Haesebrouck Freddy
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):133-136. doi: 10.1099/ijs.0.02206-0.
Lactic acid bacteria were isolated from the crop and intestines of pigeons. One group of strains, showing similar genomic patterns after screening with tRNA intergenic spacer PCR, could not be identified to the species level. Sequencing of the 16S rRNA gene of one representative strain revealed about 96% similarity to sequences from Lactobacillus fermentum and Lactobacillus mucosae. Determination of the DNA base composition, DNA-DNA hybridization experiments, SDS-PAGE of whole-cell proteins and biochemical testing confirmed that the seven strains studied constitute a single novel Lactobacillus species, for which the name Lactobacillus ingluviei sp. nov. is proposed. The type strain is strain KR3T (=LMG 20380T =CCUG 45722T).
从鸽子的嗉囊和肠道中分离出乳酸菌。一组菌株在用tRNA基因间隔区PCR筛选后显示出相似的基因组模式，但无法鉴定到种水平。对一株代表性菌株的16S rRNA基因进行测序，发现其与发酵乳杆菌和黏膜乳杆菌的序列相似度约为96%。DNA碱基组成测定、DNA-DNA杂交实验、全细胞蛋白的SDS-PAGE和生化测试证实，所研究的七株菌株构成一个单一的新型乳杆菌物种，为此提出了“嗉囊乳杆菌（Lactobacillus ingluviei sp. nov.）”这一名称。模式菌株是KR3T（=LMG 20380T =CCUG 45722T）。
