Giblin-Davis R M, Williams D S, Bekal S, Dickson D W, Brito J A, Becker J O, Preston J F
University of Florida - IFAS, Fort Lauderdale Research and Education Center, 3205 College Avenue, Fort Lauderdale, FL 33314-7719, USA.
Microbiology and Cell Science, University of Florida, PO Box 110700, Gainesville, FL 32611-0700, USA.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):197-200. doi: 10.1099/ijs.0.02292-0.
Taxonomically relevant characteristics of a fastidiously Gram-positive, obligately endoparasitic prokaryote (strain S-1) that uses the phytoparasitic sting nematode Belonolaimus longicaudatus as its host are reviewed. 16S rDNA sequence similarity (> or = 93%) confirms its congeneric ranking with other Pasteuria species and strains from nematodes and cladocerans and corroborates morphological, morphometric and host range evidence suggesting a novel taxon. The 16S rDNA sequence of strain S-1 has greatest similarity (96%) to the 16S rDNA sequences of both Pasteuria penetrans from root-knot nematodes (Meloidogyne species) and the recently reported strain of Pasteuria isolated from the soybean cyst nematode Heterodera glycines. Because the obligately endoparasitic nature of prokaryotes in the genus Pasteuria prevents isolation of definitive type strains, strain S-1 is proposed as 'Candidatus Pasteuria usgae' sp. nov.
对一种苛求性革兰氏阳性、专性内寄生原核生物（菌株S-1）的分类学相关特征进行了综述，该原核生物以植物寄生性刺痛线虫长针线虫（Belonolaimus longicaudatus）为宿主。16S rDNA序列相似性（≥93%）证实了它与来自线虫和枝角类动物的其他巴氏杆菌属物种及菌株同属一类，并佐证了形态学、形态测量学和宿主范围证据，表明这是一个新的分类单元。菌株S-1的16S rDNA序列与来自根结线虫（Meloidogyne属物种）的穿刺巴氏杆菌（Pasteuria penetrans）以及最近报道的从大豆胞囊线虫（Heterodera glycines）分离出的巴氏杆菌菌株的16S rDNA序列具有最高的相似性（96%）。由于巴氏杆菌属中原核生物的专性内寄生性质使得无法分离出确定的模式菌株，因此提议将菌株S-1定为“暂定巴斯德氏菌大豆亚种”（‘Candidatus Pasteuria usgae’ sp. nov.）。
