Revisiting the Life-Cycle of Infecting under Soil-Less Medium, and Effect of Streptomycin Sulfate on its Development.

Pasteuria penetrans is a Gram-positive, endospore forming soil bacterium, infecting root-knot nematodes, Meloidogyne spp. Being obligate in nature, the bacterium is not easily grown in vitro, and the in vivo culturing technique is relied on the soil-based microcosm since long. Hence, culturing of P. penetrans using CYG germination pouches as a soil-less medium for plant growth, promises to provide a contamination free environment along with ease in isolation of infected females from the plant roots. Additionally, this method increases the percentage of P. penetrans infected nematode females as compared with the soil-based system. Schematic observation of all the life stages of P. penetrans was documented, which revealed chronological fragmentation of vegetative microcolony inside the nematode body demonstrating the formation of some stages not reported earlier. Further, germination of endospores attached to infective juveniles was found to be most likely asynchronous as single female nematode contained most of the developing stages of P. penetrans concurrently. Additionally, the effect of an antibiotic, streptomycin sulfate was evaluated for effects on the growth and development of the bacterium at different concentrations. Higher doses of antibiotic were found to exert a negative impact on the development of P. penetrans , which shows the incompatibility of Pasteuria and streptomycin sulfate.