Ajithkumar Bindu, Ajithkumar Vasudevan P, Iriye Ryozo, Doi Yukio, Sakai Tadashi
Ecological and Toxicological Laboratory, Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University (United Graduate School of Agriculture, Gifu University), 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan.
UltraStructure Research Laboratories Ltd, Tochiku, Yokohama 224-0007, Japan.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):253-258. doi: 10.1099/ijs.0.02158-0.
A strain (KREDT) that formed endospores and produced the pigment prodigiosin was isolated from activated sludge. The presence of spores in cells of strain KREDT was evident upon electron microscopy examination, heat treatment and the detection of dipicolinic acid in the cells. Biochemical characteristics, and 16S rDNA sequence and DNA-DNA homology data identified strain KREDT as Serratia marcescens. The major respiratory quinone of strain KREDT was found to be ubiquinone Q-8. The formation of endospores by Gram-negative bacteria has not been observed previously, and has never been reported in any species of Serratia. Here, it is shown that strain KREDT (JCM 11315T = CIP 107489T) represents a novel subspecies of S. marcescens, for which the name Serratia marcescens subsp. sakuensis is proposed.
从活性污泥中分离出了一种能形成芽孢并产生灵菌红素的菌株（KREDT）。通过电子显微镜检查、热处理以及检测细胞中的吡啶二羧酸，发现菌株KREDT的细胞中存在芽孢。生化特性、16S rDNA序列和DNA-DNA同源性数据将菌株KREDT鉴定为粘质沙雷氏菌。发现菌株KREDT的主要呼吸醌为泛醌Q-8。此前尚未观察到革兰氏阴性菌形成芽孢的现象，在任何沙雷氏菌属物种中也从未有过报道。在此，研究表明菌株KREDT（JCM 11315T = CIP 107489T）代表了粘质沙雷氏菌的一个新亚种，为此提出了粘质沙雷氏菌萨库埃亚种（Serratia marcescens subsp. sakuensis）这一名称。
