Fujii Katsuhiko, Satomi Masataka, Morita Naoki, Motomura Taizo, Tanaka Tadaharu, Kikuchi Shintaro
Department of Applied Chemistry, Muroran Institute of Technology, Mizumoto, Muroran 050-8585, Japan.
Food Processing Division, National Research Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama 236-8648, Japan.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):47-52. doi: 10.1099/ijs.0.02301-0.
An oestradiol-degrading bacterium isolated at a sewage treatment plant in Tokyo was studied phenotypically, genotypically and phylogenetically. Analysis of its 16S rDNA sequence, DNA base composition, whole-cell fatty acid profile and isoprenoid quinone composition, as well as the presence of sphingoglycolipid, revealed that the isolate is a member of the genus Novosphingobium. However, the sequence similarity of its 16S rDNA to those of known Novosphingobium species was no higher than 97%, implying that the isolate is distinctive. The results of DNA-DNA hybridization experiments and physiological characterization also indicated that the isolate represents a novel Novosphingobium species, for which the name Novosphingobium tardaugens sp. nov. is proposed; strain ARI-1T (=JCM 11434T =ATCC BAA-531T =IFO 16725T) is the type strain.
对从东京某污水处理厂分离出的一株雌二醇降解菌进行了表型、基因型和系统发育分析。对其16S rDNA序列、DNA碱基组成、全细胞脂肪酸谱和类异戊二烯醌组成以及鞘糖脂的存在情况进行分析后发现，该分离株属于新鞘氨醇杆菌属。然而，其16S rDNA与已知新鞘氨醇杆菌属物种的序列相似性不高于97%，这表明该分离株具有独特性。DNA-DNA杂交实验和生理特性分析结果也表明，该分离株代表一个新的新鞘氨醇杆菌属物种，为此提出新名称新鞘氨醇杆菌迟缓降解菌（Novosphingobium tardaugens sp. nov.）；菌株ARI-1T（=JCM 11434T =ATCC BAA-531T =IFO 16725T）为模式菌株。
