Bednenko Janna, Cingolani Gino, Gerace Larry
Department of Cell, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA.
Traffic. 2003 Mar;4(3):127-35. doi: 10.1034/j.1600-0854.2003.00109.x.
Nucleocytoplasmic transport is mediated by shuttling receptors that recognize specific signals on protein or RNA cargoes and translocate the cargoes through the nuclear pore complex. Transport receptors appear to move through the nuclear pore complex by facilitated diffusion, involving repeated cycles of binding to and dissociation from nucleoporins with phenylalanine-glycine motifs. We discuss recent experimental approaches and results that have begun to provide molecular insight into the mechanisms by which transport complexes traverse the nuclear pore complex, and point out the significant gaps in understanding that remain.
核质运输由穿梭受体介导，这些受体识别蛋白质或RNA货物上的特定信号，并通过核孔复合体转运货物。运输受体似乎通过易化扩散穿过核孔复合体，涉及与具有苯丙氨酸 - 甘氨酸基序的核孔蛋白反复结合和解离的循环。我们讨论了最近的实验方法和结果，这些方法和结果已开始为运输复合体穿越核孔复合体的机制提供分子层面的见解，并指出了理解上仍然存在的重大差距。
