Yagasaki Hiroki, Kawata Nozomu, Takimoto Yukie, Nemoto Norimichi
Department of Urology, Nihon University School of Medicine, 30-1 Oyaguchi Kamichou, Itabashi-ku, Tokyo 173-0032, Japan.
Int J Urol. 2003 Apr;10(4):220-7. doi: 10.1046/j.0919-8172.2003.00608.x.
The present study was carried out to clarify whether a histopathological analysis of vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-beta1) and matrix metalloproteinase 2 (MMP-2) can help predict the outcome of renal cell carcinoma (RCC). We examined the expression of VEGF, TGF-beta1 and MMP-2 in a large series of RCC with a long follow-up, based on histopathological factors and survival.
Immunostaining for VEGF, TGF-beta1 and MMP-2 was performed on formalin-fixed, paraffin-embedded tissue sections from 84 patients with RCC who underwent nephrectomy at our institution between 1985 to 2000. The microvessel density (MVD) of tumor tissue was measured after it immunohistochemically stained with CD105 (Endoglin) monoclonal antibody.
A significant association was observed in the expression of VEGF and TGF-beta1 regarding the stage (P < 0.01, P < 0.01), nuclear grade (P < 0.01, P < 0.01) and MVD (P < 0.001, P < 0.001), respectively. However, no correlation was found among the results of MMP-2, nuclear grade and MVD. A multivariate analysis demonstrated both the nuclear grade and MVD to be independent prognostic factors.
Our results suggested that the expression of both VEGF and/or TGF-beta1 can be useful predictive prognostic factors RCC. In addition, a multivariate analysis demonstrated MVD to be an independent prognostic factor of RCC.
本研究旨在阐明对血管内皮生长因子（VEGF）、转化生长因子-β1（TGF-β1）和基质金属蛋白酶2（MMP-2）进行组织病理学分析是否有助于预测肾细胞癌（RCC）的预后。我们基于组织病理学因素和生存率，对一大系列经过长期随访的RCC病例中VEGF、TGF-β1和MMP-2的表达情况进行了检测。
对1985年至2000年间在本机构接受肾切除术的84例RCC患者的福尔马林固定、石蜡包埋组织切片进行VEGF、TGF-β1和MMP-2的免疫染色。用CD105（内皮糖蛋白）单克隆抗体对肿瘤组织进行免疫组化染色后，测量肿瘤组织的微血管密度（MVD）。
分别观察到VEGF和TGF-β1的表达与分期（P<0.01，P<0.01）、核分级（P<0.01，P<0.01）和MVD（P<0.001，P<0.001）之间存在显著相关性。然而，MMP-2的结果与核分级和MVD之间未发现相关性。多因素分析表明核分级和MVD均为独立的预后因素。
我们的结果表明，VEGF和/或TGF-β1的表达可能是RCC有用的预测性预后因素。此外，多因素分析表明MVD是RCC的独立预后因素。
