血管内皮生长因子（VEGF）的免疫组化表达与肾细胞癌中的微血管密度不相关。

Immunohistochemical expression of vascular endothelial growth factor (VEGF) does not correlate with microvessel density in renal cell carcinoma.

作者信息

Raica M, Cimpean A M, Anghel A

机构信息

Department of Histology & Cytology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.

出版信息

Neoplasma. 2007;54(4):278-84.

PMID:17822316

Abstract

The aim of present study was to investigate the relationship between the immunohistochemical expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) assessed by CD31 and endoglin (CD105) in renal cell carcinoma (RCC). Specimens from 45 cases of RCC. were formalin-fixed, paraffin embedded, and sections were stained with H&E. Additional sections from each case were stained for VEGF, CD31, CD105, and alpha smooth muscle cell actin (SMA). VEGF immunohistochemical expression was estimated as negative (0), weak positive (+1), moderate positive (+2), and intense positive (+3). Microvessel density (MVD) was estimated on 5 hot spots (x400) from each case, and the arithmetic media was the final result. MVD was separately calculated on slides stained with CD31 and CD105. The rate between mature and immature blood vessels was calculated on slides stained with CD31/CD105/SMA. Statistic analysis was performed with SPSS10.0. The immunoreaction for VEGF was positive in epithelial cells of the renal tubules, and occasionally, in endothelial cells. In RCC, tumor cells were positive in 34 from 45 cases (75.5%). 11 cases were negative, 14 were slightly positive (+1), 13 moderate (+2), and 7 intense (+3). No relationship was found between the expression of VEGF and pathological form and nuclear grade, excepting for the chromophilic variant (3 cases, all positive). CD31 was positive in all cases, and CD105 in 39 cases. The mean values of MVD on slides stained for CD31 and CD105 were: 31.68 (range 9.8-60.2)/20.66 (range 4.2-52.8). The rate CD31/SMA positive blood vessels was 1/0.62. VEGF was expressed in 75.5% of 45 cases with RCC, and the mean value of MVD CD31/CD105 was 31.68/20.66. The immunohistochemical expression of VEGF does not correlate with MVD performed on slides stained for both CD31 and endoglin. The majority of blood vessels in the tumor area are of mature type, with perivascular cells positive for SMA.

摘要

本研究的目的是探讨肾细胞癌（RCC）中血管内皮生长因子（VEGF）的免疫组化表达与通过CD31和内皮糖蛋白（CD105）评估的微血管密度（MVD）之间的关系。收集45例肾细胞癌标本，用福尔马林固定、石蜡包埋，切片进行苏木精-伊红（H&E）染色。每例标本的其他切片分别进行VEGF、CD31、CD105和α平滑肌肌动蛋白（SMA）染色。VEGF免疫组化表达评估为阴性（0）、弱阳性（+1）、中度阳性（+2）和强阳性（+3）。从每例标本的5个热点区域（×400）估计微血管密度（MVD），取算术平均值作为最终结果。分别在CD31和CD105染色的玻片上计算MVD。在CD31/CD105/SMA染色的玻片上计算成熟血管与未成熟血管的比例。使用SPSS10.0进行统计学分析。VEGF的免疫反应在肾小管上皮细胞中呈阳性，偶尔在内皮细胞中也呈阳性。在肾细胞癌中，45例中有34例（75.5%）肿瘤细胞呈阳性。11例为阴性，14例为弱阳性（+1），13例为中度阳性（+2），7例为强阳性（+3）。除嗜色变体（3例，均为阳性）外，未发现VEGF表达与病理类型和核分级之间存在相关性。所有病例CD31均呈阳性，39例CD105呈阳性。CD31和CD105染色玻片上MVD的平均值分别为：31.68（范围9.8 - 60.2）/20.66（范围4.2 - 52.8）。CD31/SMA阳性血管的比例为1/0.62。45例肾细胞癌中有75.5%表达VEGF，CD31/CD105的MVD平均值为31.68/20.66。VEGF的免疫组化表达与在CD31和内皮糖蛋白染色玻片上进行的MVD无关。肿瘤区域的大多数血管为成熟型，血管周围细胞SMA呈阳性。