Sato Y, Kobayashi Y, Sasaki H, Toyama T, Kondo S, Kiriyama M, Fujii Y
Department of Surgery II, Nagoya City University Medical School, Japan.
Eur J Surg Oncol. 2003 Apr;29(3):284-7. doi: 10.1053/ejso.2002.1412.
Inhibitors of DNA binding 2 (Id2) has been reported to be overexpressed in neuroblastoma cell lines carrying extra copies of the N-myc gene. It has been suggested that Id2 may be involved in the disturbed regulation of cell cycle by interfering with retinoblastoma protein.
In this report we assessed Id2 gene expression in 20 neuroblastoma samples and eight normal ganglion tissues using quantitative reverse transcription-PCR (RT-PCR). Id2 expression in resected clinical samples of neuroblastoma needs to be studied.
Id2 messenger RNA (mRNA) was expressed in all the neuroblastoma samples. The level of Id2 mRNA expression was not influenced by the patient's age, gender, tumor's clinical stage, DNA ploidy pattern, histological pattern, the level of N-myc mRNA expression and whether the patient was found by mass screening or by symptom. Id2 was also expressed in comparable levels in normal differentiated sympathetic ganglion in adult.
Our data suggest that Id2's role in the oncogenesis or progression of neuroblastoma is minimal.
据报道，在携带N - myc基因额外拷贝的神经母细胞瘤细胞系中，DNA结合抑制因子2（Id2）过表达。有人提出，Id2可能通过干扰视网膜母细胞瘤蛋白而参与细胞周期的调节紊乱。
在本报告中，我们使用定量逆转录聚合酶链反应（RT - PCR）评估了20个神经母细胞瘤样本和8个正常神经节组织中Id2基因的表达。需要研究神经母细胞瘤切除临床样本中的Id2表达。
Id2信使核糖核酸（mRNA）在所有神经母细胞瘤样本中均有表达。Id2 mRNA表达水平不受患者年龄、性别、肿瘤临床分期、DNA倍体模式、组织学模式、N - myc mRNA表达水平以及患者是通过大规模筛查还是症状发现的影响。Id2在成人正常分化的交感神经节中也以相当的水平表达。
我们的数据表明，Id2在神经母细胞瘤的发生或进展中的作用极小。
