邻苯二甲酸二丁酯和邻苯二甲酸二（2-乙基己基）酯对鲤鱼（Cyprinus carpio L.）吞噬细胞的体外调节作用
In vitro modulation of common carp (Cyprinus carpio L.) phagocytic cells by Di-n-butyl phthalate and Di-2-ethylhexyl phthalate.
作者信息
Watanuki Hironobu, Gushiken Yoshiki, Sakai Masahiro
机构信息
United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, 1-21-24, 890-0065, Kagoshima, Japan.
出版信息
Aquat Toxicol. 2003 Apr 10;63(2):119-26. doi: 10.1016/s0166-445x(02)00172-8.
The modulation of common carp, Cyprinus carpio L. phagocytic cells by Di-n-butyl phthalate (DBP) and Di-2-ethylhexyl phthalate (DEHP) were analyzed in vitro. Carp head kidney leucocytes were cultured in a RPMI 1640 medium containing DBP or DEHP at concentration of 1-1000 nM and the production of superoxide anion was measured via the in vitro reduction of nitroblue tetrazolium (NBT). Macrophages incubated with 100 up to 1000 nM of DBP showed an increase in their production of superoxide anion in comparison with the control macrophages which, were cultured without DBP. DEHP also increased the production of superoxide anion in phagocytic cells from carp kidneys. Phagocytic cells treated with 10 and 100 ng of DEHP showed increased phagocytic activity. DBP and DEHP also significantly increased the production of nitric oxide in carp macrophages. These results showed that DBP and DEHP modulate the function of phagocytic cells in vitro.
在体外分析了邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）对鲤（Cyprinus carpio L.）吞噬细胞的调节作用。将鲤头肾白细胞培养于含有浓度为1-1000 nM DBP或DEHP的RPMI 1640培养基中，并通过体外还原氮蓝四唑（NBT）来测定超氧阴离子的产生。与未用DBP培养的对照巨噬细胞相比，用100至1000 nM DBP孵育的巨噬细胞超氧阴离子产生增加。DEHP也增加了鲤肾吞噬细胞中超氧阴离子的产生。用10和100 ng DEHP处理的吞噬细胞吞噬活性增加。DBP和DEHP也显著增加了鲤巨噬细胞中一氧化氮的产生。这些结果表明，DBP和DEHP在体外调节吞噬细胞的功能。
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