Saika Shizuya, Miyamoto Takeshi, Ishida Iku, Ohnishi Yoshitaka, Ooshima Akira
Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan.
Invest Ophthalmol Vis Sci. 2003 Apr;44(4):1622-8. doi: 10.1167/iovs.02-0873.
To examine whether tissues of human capsular opacification and subcapsular cataract contain osteopontin, an adhesive matrix protein, and whether mouse lens epithelium expresses osteopontin after injury.
An immunohistochemical examination was conducted to determine whether matrices in human postoperative capsular specimens and anterior subcapsular cataract contain osteopontin. The spatial and temporal protein expression patterns of osteopontin were then determined in epithelium of a healing mouse lens after a capsular incision.
Human lens epithelial cells in the specimens extracted at the time of vitrectomy 10 days after cataract surgery and also after longer healing intervals were labeled with an anti-osteopontin antibody, whereas uninjured lens epithelium was not. In the later healing phase, matrix of capsular opacification was positive for osteopontin. Lens cells amid anterior subcapsular cataract tissue were also positive. Osteopontin was detected in the cell surface and membrane and the cytoplasm of lens cells, as well as in the matrix. Unlike normal uninjured specimens, anterior lens capsule of some of the healing postoperative specimens and anterior subcapsular cataract specimens also faintly or weakly stained for osteopontin. Mouse lens epithelium started to express osteopontin protein at 8 hours after injury, before the cells changed their shape from epithelial cell type to fibroblast type. Expression of osteopontin lasted during the healing interval, even after the cells transformed into fibroblast-like cells.
Extracellular matrix in human postoperative capsular opacification and anterior subcapsular cataract contains osteopontin. Epithelial cells of a mouse lens also ectopically express osteopontin in response to capsular injury.
研究人类晶状体后囊膜混浊及晶状体后囊下白内障组织中是否含有骨桥蛋白（一种黏附性基质蛋白），以及小鼠晶状体上皮细胞在损伤后是否表达骨桥蛋白。
进行免疫组织化学检查，以确定人类术后晶状体囊膜标本和晶状体前囊下白内障中是否含有骨桥蛋白。然后确定小鼠晶状体囊膜切开后愈合过程中骨桥蛋白在晶状体上皮细胞中的时空蛋白表达模式。
白内障手术后10天玻璃体切除时以及更长愈合时间后提取的标本中的人类晶状体上皮细胞，用抗骨桥蛋白抗体标记呈阳性，而未受伤的晶状体上皮细胞则未被标记。在愈合后期，晶状体后囊膜混浊的基质骨桥蛋白呈阳性。晶状体前囊下白内障组织中的晶状体细胞也呈阳性。在晶状体细胞的表面、细胞膜和细胞质以及基质中均检测到骨桥蛋白。与正常未受伤的标本不同，一些愈合后的术后标本和晶状体前囊下白内障标本的晶状体前囊膜骨桥蛋白染色也较淡或较弱。小鼠晶状体上皮细胞在损伤后8小时开始表达骨桥蛋白，此时细胞尚未从上皮细胞形态转变为成纤维细胞形态。在愈合期间，骨桥蛋白持续表达，即使细胞已转变为成纤维细胞样细胞。
人类术后晶状体后囊膜混浊和晶状体前囊下白内障的细胞外基质中含有骨桥蛋白。小鼠晶状体上皮细胞在受到晶状体囊膜损伤时也会异位表达骨桥蛋白。
