自发性消退型视网膜母细胞瘤眼前囊下白内障的免疫组化观察。

Immunohistochemical observation of anterior subcapsular cataract in eye with spontaneously regressed retinoblastoma.

机构信息

Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama, 641-0012, Japan.

出版信息

J Cataract Refract Surg. 2010 Mar;36(3):503-7. doi: 10.1016/j.jcrs.2009.08.042.

DOI:10.1016/j.jcrs.2009.08.042

PMID:20202553

Abstract

We report the histological findings of secondary cataract in an eye with a spontaneously regressed retinoblastoma to obtain keys to clarify the mechanism of this phenomenon. During phacoemulsification, opacified anterior capsule was obtained, fixed in formalin, and embedded in paraffin. Paraffin sections of the specimen were histologically observed. Hematoxylin-eosin staining showed extracellular matrix accumulation in the extracted fibrous anterior subcapsular opacification. Immunohistochemistry revealed the presence of fibrous collagen types and cellular fibronectin. Presumed lens cells amid matrix were positively labeled for vimentin, alpha-smooth muscle actin, and phospho-Smad2. Histology of the fibrous anterior subcapsular opacification tissue showed the possibility of epithelial-mesenchymal transition of the lens epithelial cells in the secondary cataract following a spontaneously regressed retinoblastoma.

摘要

我们报告了一例自发消退型视网膜母细胞瘤眼继发性白内障的组织学发现，以期获得阐明这一现象机制的关键。在超声乳化过程中，获得了混浊的前囊，用福尔马林固定，并包埋在石蜡中。对标本的石蜡切片进行了组织学观察。苏木精-伊红染色显示，提取的纤维性前囊下混浊中有细胞外基质的积累。免疫组织化学显示存在纤维状胶原类型和细胞纤维连接蛋白。在基质中，假定的晶状体细胞对波形蛋白、α-平滑肌肌动蛋白和磷酸化 Smad2 呈阳性标记。纤维性前囊下混浊组织的组织学显示，在自发消退型视网膜母细胞瘤后，继发性白内障的晶状体上皮细胞存在上皮-间充质转化的可能性。