Cohnen G, Augener W, Reuter A, Brittinger G
Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(5):463-8.
Peripheral blood T and B lymphocytes were determined in normal humans at different ages. Spontaneous rosette formation with sheep red blood cells (SRBC) was used as a marker for T cells. B cells were detected by immunofluorescent staining of membrane-bound immunoglobulins. Blood samples from old individuals contained significantly lower T lymphocyte numbers than those from children. This diminution of circulating T cells caused a reduction of the total lymphocyte count in the elderly persons. No significant differences were between the T cell values of young and old adults. Whereas the percentages of B cells indicated an increase of this lymphocyte population in old humans, the absolute numbers of B lymphocytes remained almost unchanged during aging.
对不同年龄段的正常人外周血T淋巴细胞和B淋巴细胞进行了测定。以与绵羊红细胞（SRBC）形成自发玫瑰花结作为T细胞的标志物。通过膜结合免疫球蛋白的免疫荧光染色检测B细胞。老年个体的血样中T淋巴细胞数量明显低于儿童。循环T细胞的减少导致老年人总淋巴细胞计数降低。青年人和老年人的T细胞值无显著差异。虽然B细胞百分比表明老年人体内这种淋巴细胞群体增加，但B淋巴细胞的绝对数量在衰老过程中几乎保持不变。
