采用胶束电动色谱中的扫集技术，通过毛细管电泳/荧光光谱法测定小鼠血液中的麦角酸二乙酰胺（LSD）。

Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography.

作者信息

Fang Ching, Liu Ju-Tsung, Chou Shiu-Huey, Lin Cheng-Huang

机构信息

Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan.

出版信息

Electrophoresis. 2003 Mar;24(6):1031-7. doi: 10.1002/elps.200390119.

DOI:10.1002/elps.200390119

PMID:12658692

Abstract

The separation and on-line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on-line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were applied; the optimum on-line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; approximately 1/10 to the value of LD(50)). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.

摘要

采用十二烷基硫酸钠（SDS）作为表面活性剂，通过毛细管电泳/荧光光谱法实现了小鼠血液中麦角酸二乙酰胺（LSD）的分离和在线富集。应用了包括扫集胶束电动色谱法（sweeping-MEKC）和阳离子选择性完全进样-扫集胶束电动色谱法（CSEI-sweep-MEKC）在内的在线样品富集技术；确定了最佳的在线富集和分离条件。在实际样品分析中，在一只受试小鼠的血液样本中发现了LSD（给一只20 g小鼠喂食0.1 mg LSD；约为半数致死量（LD(50)）值的1/10）。结果，在摄入剂量后20分钟和60分钟时，分别检测到120 ng/mL和30 ng/mL的LSD。