Department of Mechanical & Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.
Electrophoresis. 2012 Aug;33(16):2570-6. doi: 10.1002/elps.201200209.
Micellar electrokinetic chromatography (MEKC), combined with on-line concentration techniques, cation-selective exhaustive injection (CSEI) and sweeping, was developed for the analysis of cotinine, the primary biomarker for exposure to secondhand smoke. Experimental parameters including sample matrix, surfactant concentration, injection length and concentration of high-conductivity buffer, and sample electrokinetic injection time were optimized for electrophoretic enrichment and separation processes. Under the optimal conditions, the detection sensitivity of cotinine was enhanced by about 5000-fold using CSEI-sweeping MEKC compared to normal MEKC. The limit of detection for cotinine was found to be 0.2 ng/mL using ultraviolet absorbance detection. Furthermore, the developed method was successfully applied to the detection of cotinine in mouse serum samples.
胶束电动色谱（MEKC）与在线浓缩技术、阳离子选择性完全进样（CSEI）和扫集相结合，被开发用于分析可替宁，这是暴露于二手烟的主要生物标志物。实验参数包括样品基质、表面活性剂浓度、进样长度和高导电性缓冲液浓度以及样品电动进样时间，用于电泳富集和分离过程。在最佳条件下，与普通 MEKC 相比，CSEI-扫集 MEKC 将可替宁的检测灵敏度提高了约 5000 倍。使用紫外吸收检测，可替宁的检测限为 0.2 ng/mL。此外，所开发的方法成功应用于检测小鼠血清样品中的可替宁。
