Huang Qiong, Hu Yanhua, Jiang Fagang, Chen Hong
Department of Ophthalmology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
J Huazhong Univ Sci Technolog Med Sci. 2002;22(1):62-5. doi: 10.1007/BF02904792.
To investigate whether the TGF-beta 1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-beta 1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-beta 1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-beta 1 protein expression specific for pMAMTGF-beta 1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-beta 1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.
为研究脂质体携带的转化生长因子β1（TGF-β1）质粒DNA能否导入培养的兔角膜上皮细胞，对质粒pMAM TGF-β1在培养的角膜上皮细胞中的特异性表达进行了研究。将脂质体介导的pMAMT-GF-β1转染培养的角膜上皮细胞12小时后两天，采用免疫组织化学染色法检测细胞中pMAMTGF-β1特异性的TGF-β1蛋白表达，阳性率为23.37%。结果表明，脂质体可有效地将外源质粒DNA导入培养的兔角膜上皮细胞，这将为研究TGF-β1在角膜上皮细胞相关生理和病理机制方面提供一种有前景的方法。
