Honma Y, Nishida K, Sotozono C, Kinoshita S
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto, 602, Japan.
Exp Eye Res. 1997 Sep;65(3):391-6. doi: 10.1006/exer.1997.0338.
Corneal epithelial wound healing is intimately controlled by a variety of growth factors, such as epidermal growth factor (EGF), keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), and transforming growth factor-betas (TGF-betas). In this study, we investigate the effects of TGF-beta1 and TGF-beta2 on cultured rabbit corneal epithelial cell proliferation promoted by EGF, KGF, or HGF. Both TGF-beta1 and -beta2 dose-dependently inhibited corneal epithelial cell proliferation promoted by KGF (40 ng ml-1) and HGF (40 ng ml-1), and weakly inhibited cell proliferation promoted by EGF (4 ng ml-1). Furthermore, the inhibitory effect tended to be stronger with TGF-beta2 than TGF-beta1. These findings suggest that TGF-beta1 and -beta2 play important roles as negative modulators against the cell proliferation effect of EGF, KGF and HGF.
角膜上皮伤口愈合受到多种生长因子的密切调控，如表皮生长因子（EGF）、角质形成细胞生长因子（KGF）、肝细胞生长因子（HGF）和转化生长因子-β（TGF-β）。在本研究中，我们调查了TGF-β1和TGF-β2对由EGF、KGF或HGF促进的培养兔角膜上皮细胞增殖的影响。TGF-β1和-β2均呈剂量依赖性地抑制由KGF（40 ng/ml）和HGF（40 ng/ml）促进的角膜上皮细胞增殖，并微弱抑制由EGF（4 ng/ml）促进的细胞增殖。此外，TGF-β2的抑制作用往往比TGF-β1更强。这些发现表明，TGF-β1和-β2作为针对EGF、KGF和HGF的细胞增殖作用的负调节剂发挥重要作用。
