Ma Yan-lin, Li Qi, Pan Qian
National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Hunan Yi Ke Da Xue Xue Bao. 2002 Dec 28;27(6):487-90.
To develop an efficient, rapid and reliable method for sex determination in preimplantation genetic diagnosis.
Nested PCR and fluorescent PCR were used to amplify amelogenin and IL-3 genes from human single lymphocytes and blastomeres.
The amplification efficiencies of nested PCR and fluorescent PCR of man single lymphocyte/woman single lymphocyte/blastomere were 64.7%/65%/85.7%, and 72%/76%/86.9% respectively. The PCR products of the lymphocyte were exactly the same as those of the donor gDNA. The PCR products of blastomeres coming from the same embryo were all identical.
The amplification efficiencies of nested PCR and fluorescent PCR are similar while fluorescent PCR is more conventional, time sparing and sensitive.
开发一种用于植入前基因诊断中性别鉴定的高效、快速且可靠的方法。
采用巢式聚合酶链反应（Nested PCR）和荧光定量聚合酶链反应（荧光PCR）从人单个淋巴细胞和卵裂球中扩增牙釉蛋白和白细胞介素-3基因。
男性单个淋巴细胞/女性单个淋巴细胞/卵裂球的巢式PCR和荧光PCR扩增效率分别为64.7%/65%/85.7%和72%/76%/86.9%。淋巴细胞的PCR产物与供体基因组DNA的产物完全相同。来自同一胚胎的卵裂球的PCR产物均相同。
巢式PCR和荧光PCR的扩增效率相似，而荧光PCR更简便、省时且灵敏。
