Perrin A, Duborjal H, Roudier E, Massarelli R
Unite de Biophysique Cellulaire et Moléculaire, CRSSA, BP 87, 38702 La Tronche, France.
Arch Ital Biol. 2003 Feb;141(1):1-10.
The metabolism of [1-13C] glucose was followed in C6 rat glioma cells immobilized on a gel thread and in perchloric extracts of the same cells in culture. The results showed that the main metabolite of [1-13C] glucose is [3-13C] lactate. The effects of hypoxia were followed in the perchloric acid extracts of C6 cells. In normoxic conditions, the main metabolites produced by the cells were [3-'3C] lactate, [3-13C] alanine, [2-13C], [3-13C] and [4-13C] glutamate. Lactate newly synthesized from glucose appeared to be exported in the perfusion medium when living cells were immobilized in gel threads made of extracellular matrix. After 5 h of hypoxia, the lactate labelling measured in PCA cell extracts was increased that of glutamate decreased and the appearance of a spectral line at 66.01 ppm, identified as [1-13C] glycerol-3-phosphate, was observed. The data suggest that the synthesis of glycerol-3-phosphate in these cells might represent a sign of hypoxia.
对固定在凝胶丝上的C6大鼠胶质瘤细胞及其培养的相同细胞的高氯酸提取物中[1-¹³C]葡萄糖的代谢进行了追踪。结果表明，[1-¹³C]葡萄糖的主要代谢产物是[3-¹³C]乳酸。在C6细胞的高氯酸提取物中研究了缺氧的影响。在常氧条件下，细胞产生的主要代谢产物是[3-¹³C]乳酸、[3-¹³C]丙氨酸、[2-¹³C]、[3-¹³C]和[4-¹³C]谷氨酸。当活细胞固定在由细胞外基质制成的凝胶丝中时，由葡萄糖新合成的乳酸似乎被分泌到灌注培养基中。缺氧5小时后，在PCA细胞提取物中测得的乳酸标记增加，谷氨酸标记减少，并且观察到在66.01 ppm处出现一条光谱线，鉴定为[1-¹³C]甘油-3-磷酸。数据表明，这些细胞中甘油-3-磷酸的合成可能是缺氧的一个标志。
