Jiang Chongqiu, He Fang
Department of Chemistry, Shandong Normal University, 250014, Jinan, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2003 Apr;59(6):1321-8. doi: 10.1016/s1386-1425(02)00307-4.
A new fluorescent reagent, 2-hydroxy-1-naphthaldehydene-8-aminoquinoline (HNAAQ), was synthesized. The fluorescent reaction of this reagent with beryllium was also studied. Based on this chelation, a highly sensitive spectrofluorimetric method was developed for determination of trace amounts of beryllium at pH 9.2. Under these conditions, the Be-HNAAQ complex has excitation and emission maxima at 410 and 450 nm, respectively. The linear range of the method is from 0 to 35 microg l(-1) and detection limit is 0.099 microg l(-1) of beryllium. Interference of other ions was studied. It is necessary to remove the interfering cations through concealing by EDTA and extraction separation techniques. The selectivity of the method can be increased remarkably. The procedure can be easily performed and affords good precision and accuracy. This method has been successfully applied to the determination of beryllium in mineral water and human's hair.
合成了一种新型荧光试剂2-羟基-1-萘甲醛-8-氨基喹啉（HNAAQ）。研究了该试剂与铍的荧光反应。基于这种螯合作用，建立了一种高灵敏度的荧光分光光度法，用于在pH 9.2条件下测定痕量铍。在此条件下，Be-HNAAQ络合物的激发和发射最大值分别在410和450 nm处。该方法的线性范围为0至35 μg l(-1)，铍的检测限为0.099 μg l(-1)。研究了其他离子的干扰情况。有必要通过EDTA掩蔽和萃取分离技术去除干扰阳离子。该方法的选择性可显著提高。该方法操作简便，具有良好的精密度和准确度。该方法已成功应用于矿泉水中铍和人发中铍的测定。
