Li Jing, He Fang, Jiang Chong-qiu
Department of Chemistry, Shandong Normal University, Jinan, China.
Anal Sci. 2006 Apr;22(4):607-11. doi: 10.2116/analsci.22.607.
A new fluorescent reagent, 2-hydroxy-1-naphthaldehydene-8-aminoquinoline (HNAAQ), was synthesized. The fluorescent reaction of this reagent with mercury was also studied. Based on this chelation, a highly sensitive spectrofluorometric method was developed for the determination of trace amounts of mercury in a water-ethanol (5 + 1, v/v) medium at pH 8.0. Under these conditions, the Hg-HNAAQ complex has excitation and emission maxima at 406 and 445 nm, respectively. The linear range of the method is from 0 to 16 microg L(-1) and the detection limit is 0.056 microg L(-1) of mercury. The interference of other ions was studied. In order to enhance the selectivity in the determination of mercury by the present method, we also applied the separation of mercury by distillation. Thus, the selectivity of the method could be increased remarkably. The procedure can be easily performed, and affords good precision and accuracy. This method has been successfully applied to the determination of mercury in waste water and prawns.
合成了一种新型荧光试剂2-羟基-1-萘甲醛缩8-氨基喹啉（HNAAQ）。研究了该试剂与汞的荧光反应。基于这种螯合作用，建立了一种高灵敏度的荧光分光光度法，用于在pH 8.0的水-乙醇（5 + 1，v/v）介质中测定痕量汞。在这些条件下，Hg-HNAAQ络合物的激发和发射最大值分别在406和445 nm处。该方法的线性范围为0至16 μg L⁻¹，汞的检测限为0.056 μg L⁻¹。研究了其他离子的干扰。为了提高本方法测定汞的选择性，还采用了蒸馏法分离汞。因此，该方法的选择性可显著提高。该方法操作简便，具有良好的精密度和准确度。该方法已成功应用于废水和对虾中汞的测定。
