Petrovic L, Schlegel K A, Ries J, Park J, Diebel E, Schultze-Mosgau S, Wiltfang J
Klinik und Poliklinik für Mund-; Kiefer- und Gesichtschirurgie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Glückstrasse 11, 91054 Erlangen.
Mund Kiefer Gesichtschir. 2003 Mar;7(2):102-7. doi: 10.1007/s10006-003-0452-5. Epub 2003 Feb 14.
Taurolidine (Taurolin) is a derivative of the amino acid taurine, successfully used in the treatment of peritonitis. In vitro and in vivo studies have shown that taurolidine inhibits cell proliferation and induces apoptosis in a variety of tumor cell lines. At present there are no published studies on the use of taurolidine in the treatment of squamous cell carcinoma. Our aim was to examine the inhibition of cell proliferation and induction of apoptosis in cell lines SCC 4 and SCC 15 treated with taurolidine in concentrations of 0.01%, 0.1%, and 0.5%. Analogue to the present investigations on adenocarcinoma cell lines, we used toxic antiseptic povidone iodine in the same concentration as for the reference group. Untreated cells were used as a control group. The cells were incubated with taurolidine or povidone iodine once for 2 h at 37 degrees C in 5% CO(2). Cell proliferation was assessed using WST-1 labeling kit after 3, 24, 48, 72, and 96 h. The additional measurement of cell apoptosis was examined using ELISA(PLUS) cell death detection kit and performed after 0, 24, and 48 h. The findings showed a significant inhibition of cell proliferation and induction of apoptosis in taurolidine-treated cells SCC 4 and SCC 15 in contrast to the reference group treated with povidone iodine or the untreated control group.
牛磺罗定（Taurolin）是氨基酸牛磺酸的衍生物，已成功用于治疗腹膜炎。体外和体内研究表明，牛磺罗定可抑制多种肿瘤细胞系的细胞增殖并诱导其凋亡。目前尚无关于牛磺罗定用于治疗鳞状细胞癌的公开研究。我们的目的是检测浓度为0.01%、0.1%和0.5%的牛磺罗定对SCC 4和SCC 15细胞系细胞增殖的抑制作用以及对凋亡的诱导作用。与目前对腺癌细胞系的研究类似，我们使用了与参考组相同浓度的有毒防腐剂聚维酮碘。未处理的细胞用作对照组。将细胞与牛磺罗定或聚维酮碘在37℃、5%二氧化碳环境中孵育一次，持续2小时。在3、24、48、72和96小时后，使用WST-1标记试剂盒评估细胞增殖。在0、24和48小时后，使用ELISA(PLUS)细胞死亡检测试剂盒检测细胞凋亡的额外指标。结果显示，与用聚维酮碘处理的参考组或未处理的对照组相比，经牛磺罗定处理的SCC 4和SCC 15细胞系的细胞增殖受到显著抑制，且诱导了细胞凋亡。
