Smotrova N G, Kremenchutskiĭ G N
Dniepropetrovsk State Medical Academy, 4 Oktyabrskaya Sq., Dniepropetrovsk, 49027, Ukraine.
Mikrobiol Z. 2002 Nov-Dec;64(6):28-34.
Strains of microorganisms (13) producing enzyme glucoseoxidase were isolated from various samples of soil. The ability to oxidase glucose in presence of oxygen is most expressed in representatives of genera Aureobasidium, Aspergillus, Penicillium. Media containing the KJ-starch indicator were used at primary step for isolation of microorganisms from the environment. The strain, identified as Aureobasidium pullulans (de Bary) Arnaud, isolated from soil had the greatest glucoseoxidase activity, remained active after lyophilization, grew on the medium, which contained low concentration of glucose and nitrate for candides. Use of its cells as a glucoseoxidase sensor control allowed to detect glucose in the liquids with concentration from 0.027 mM/l.
从各种土壤样本中分离出了产生葡萄糖氧化酶的微生物菌株(13种)。在有氧条件下氧化葡萄糖的能力在 Aureobasidium、Aspergillus、Penicillium 属的代表菌株中表现最为明显。在从环境中分离微生物的第一步中,使用了含有 KJ -淀粉指示剂的培养基。从土壤中分离出的被鉴定为出芽短梗霉(德巴里)阿诺德的菌株具有最大的葡萄糖氧化酶活性,冻干后仍保持活性,能在含有低浓度葡萄糖和硝酸盐的培养基上生长。将其细胞用作葡萄糖氧化酶传感器对照,能够检测浓度低至0.027 mM/l 的液体中的葡萄糖。