Viken K E
Acta Pathol Microbiol Scand C. 1976 Feb;84(1):13-22. doi: 10.1111/j.1699-0463.1976.tb03594.x.
When monocytes had differentiated to macrophages during 8 days of culture in vitro without being exposed to steroids, neither the engulfement nor the digestion step of phagocytosis was found to be influenced by cortisol or the other steroids tested. This indicates that cortisol has no direct effect on the phagocytic function of macrophages in doses below 10(-1) mg/ml. Continuous exposure to cortisol during the period of differentiation resulted in a dose dependent inhibition of the differentiation of monocytes to macrophages. Testosterone, deoxycorticosterone and tetrahydrocortisol in concentration of 10(-1) mg/ml, tested in the same way were found to be toxic to the cells. In lower concentrations, however, these steroids were found to have no effect on the cultured cells. The impaired differentiation of monocytes is suggested as an additional explanation of the reduced number of macrophages appearing at the site of inflammation during cortisol treatment.
当单核细胞在体外培养8天分化为巨噬细胞且未接触类固醇时，发现吞噬作用的吞噬步骤和消化步骤均不受皮质醇或所测试的其他类固醇的影响。这表明，剂量低于10（-1）mg/ml的皮质醇对巨噬细胞的吞噬功能没有直接影响。在分化期间持续暴露于皮质醇会导致单核细胞向巨噬细胞分化的剂量依赖性抑制。以同样方式测试的浓度为10（-1）mg/ml的睾酮、脱氧皮质酮和四氢皮质醇对细胞有毒性。然而，在较低浓度下，发现这些类固醇对培养的细胞没有影响。单核细胞分化受损被认为是皮质醇治疗期间炎症部位出现的巨噬细胞数量减少的另一种解释。
