Serafin Antonio M, Akudugu John M, Böhm Lothar
Department of Radiation Oncology, Faculty of Health Sciences and Tygerberg Hospital, PO Box 19063, Tygerberg 7505, South Africa.
Urol Res. 2003 Aug;31(4):227-31. doi: 10.1007/s00240-003-0299-0. Epub 2003 Apr 1.
The relationship between radiosensitivity and DNA repair was investigated in six human prostate cell lines, 1542-NPTX, BPH-1, 1542-CP(3)TX, 1532-CP(2)TX, 1535-CP(1)TX and LNCaP. Except for LNCaP, these cell lines are new and were derived from primary prostate tumours and normal non-tumourigenic prostate tissue. Cell survival was assessed by clonogenic assay. DNA damage was determined in non-synchronised cells by constant-field gel electrophoresis, and expressed as the fraction of DNA released. For initial damage, cells were embedded in agarose and irradiated on ice with 0-100 Gy (60)Co gamma-irradiation. Residual DNA damage was measured after 2 h and 20 h of repair. Radiosensitivity, given as the mean inactivation dose, was found to vary between 1.62 and 2.77 Gy. We found that radiosensitivity significantly correlates with the 2 h DNA repair component, giving a correlation coefficient of 0.92 ( P=0.009). In the cell lines examined here the 2 h repair component emerges as an indicator of radiosensitivity.
在六种人前列腺细胞系1542-NPTX、BPH-1、1542-CP(3)TX、1532-CP(2)TX、1535-CP(1)TX和LNCaP中研究了放射敏感性与DNA修复之间的关系。除LNCaP外，这些细胞系是新的，源自原发性前列腺肿瘤和正常非致瘤性前列腺组织。通过克隆形成试验评估细胞存活率。通过恒场凝胶电泳在非同步化细胞中测定DNA损伤，并以释放的DNA分数表示。对于初始损伤，将细胞包埋在琼脂糖中，在冰上用0-100 Gy（60）Coγ射线照射。在修复2小时和20小时后测量残留的DNA损伤。放射敏感性以平均失活剂量表示，发现其在1.62至2.77 Gy之间变化。我们发现放射敏感性与2小时DNA修复成分显著相关，相关系数为0.92（P = 0.009）。在此处检测的细胞系中，2小时修复成分成为放射敏感性的指标。
