Bayne C W, Ross M, Donnelly F, Chapman K, Buck C, Bollina P, Habib F K
University Department of Surgery, Western General Hospital, Edinburgh, UK.
Urol Int. 1998 Oct;61(1):1-7. doi: 10.1159/000030274.
Paracrine interactions between primary cultured prostate epithelial cells and stromal fibroblasts were investigated in relation to morphology, growth, androgen sensitivity and secretory activities using co-cultures in which the two populations were separated by a microporous membrane. In this new model system, both cell types maintained several aspects of the differentiated phenotype including the capacity to express 5alpha-reductase iso-enzymes and androgen receptors, to respond to androgens and to secrete prostate-specific antigen by the epithelial cells. Morphological studies demonstrated that the cells grown in co-culture exhibited round nuclei, tonofibrils and microvilli in epithelial cells and elongated nuclei, large amounts of Golgi apparatus and cilia in the fibroblasts, all indicative of the differentiated state. The co-culture system highlights the importance of the metabolic co-operation between prostate fibroblast and epithelial cells for preserving the phenotypic characteristics associated with the human prostate in vivo.
利用微孔膜将原代培养的前列腺上皮细胞和基质成纤维细胞分隔开进行共培养，研究了它们之间的旁分泌相互作用，涉及形态学、生长、雄激素敏感性和分泌活性。在这个新的模型系统中，两种细胞类型都维持了分化表型的几个方面，包括表达5α-还原酶同工酶和雄激素受体的能力、对雄激素的反应能力以及上皮细胞分泌前列腺特异性抗原的能力。形态学研究表明，共培养中生长的细胞在上皮细胞中表现出圆形细胞核、张力原纤维和微绒毛，在成纤维细胞中表现出细长细胞核、大量高尔基体和纤毛，所有这些都表明处于分化状态。共培养系统突出了前列腺成纤维细胞与上皮细胞之间代谢合作对于维持体内与人类前列腺相关的表型特征的重要性。
