Bassett D J, Fisher A B
Am J Physiol. 1976 Mar;230(3):658-63. doi: 10.1152/ajplegacy.1976.230.3.658.
Glucose metabolism was studied in isolated rat lungs ventilated with 95% O2.5% CO2 (control), 95% N2: 5% CO2 (hypoxia), and 95% CO:5% CO2 (carbon monoxide) and perfused for 100-120 min with Krebs-Ringer-bicarbonate buffer, pH 7.4, containing [U-14C] and [3-3H]glucose. The production of 14C-labeled lactate plus pyruvate (L + P) and of 14CO2 represented 48% and 22% respectively, of the total [14C]glucose utilization. The lactate-to-pyruvate ratio (L/P) was 8.7. Tritium was recovered predominantly as 3H2O in the perfusate. Wth carbon monoxide ventilation, L + P production was increased by 357% with an L/P of 52.9, and 14CO2 production was markedly decreased. A 56% decrease in lung ATP content was associated with decreased incorporation of 14C into fatty acids. Compared with CO, changes with N2 ventilation were less marked, indicating that ventilation with CO is a more effective method with which to study inhibtion of oxidative metabolism. The lung exhibits a Pasteur effectbintain ATP content or its supply for synthetic activity.
在以95% O₂、5% CO₂（对照）、95% N₂: 5% CO₂（低氧）和95% CO: 5% CO₂（一氧化碳）进行通气的离体大鼠肺中研究葡萄糖代谢。用pH 7.4的含有[U-¹⁴C]和[3-³H]葡萄糖的 Krebs-Ringer-碳酸氢盐缓冲液灌注100 - 120分钟。¹⁴C标记的乳酸加丙酮酸（L + P）和¹⁴CO₂的产生分别占总[¹⁴C]葡萄糖利用的48%和22%。乳酸与丙酮酸的比率（L/P）为8.7。灌注液中的³H主要以³H₂O的形式回收。在一氧化碳通气时，L + P的产生增加了357%，L/P为52.9，而¹⁴CO₂的产生显著减少。肺ATP含量下降56%与¹⁴C掺入脂肪酸的减少有关。与一氧化碳相比，氮气通气引起的变化不太明显，这表明一氧化碳通气是研究氧化代谢抑制的更有效方法。肺表现出巴斯德效应以维持ATP含量或其用于合成活动的供应。
