Cheng D W, Armstrong K C, Drouin G, McElroy A, Fedak G, Molnar S D
Department of Biological Sciences, Wayne State University, Biological Sciences Building, 5047 Gullen Mall, Detroit, MI 48202, USA.
Genome. 2003 Feb;46(1):119-27. doi: 10.1139/g02-111.
The DNA sequence of an extracellular (EXC) domain of an oat (Avena sativa L.) receptor-like kinase (ALrk10) gene was amplified from 23 accessions of 15 Avena species (6 diploid, 6 tetraploid, and 3 hexaploid). Primers were designed from one partial oat ALrk10 clone that had been used to map the gene in hexaploid oat to linkage groups syntenic to Triticeae chromosome 1 and 3. Cluster (phylogenetic) analyses showed that all of the oat DNA sequences amplified with these primers are orthologous to the wheat and barley sequences that are located on chromosome 1 of the Triticeae species. Triticeae chromosome 3 Lrk10 sequences were not amplified using these primers. Cluster analyses provided evidence for multiple copies at a locus. The analysis divided the ALrk EXC sequences into two groups, one of which included AA and AABB genome species and the other CC, AACC, and CCCC genome species. Both groups of sequences were found in hexaploid AACCDD genome species, but not in all accessions. The C genome group was divided into 3 subgroups: (i) the CC diploids and the perennial autotetraploid, Avena macrostachya (this supports other evidence for the presence of the C in this autotetraploid species); (ii) a sequence from Avena maroccana and Avena murphyi and several sequences from different accessions of A. sativa; and (iii) A. murphyi and sequences from A. sativa and Avena sterilis. This suggests a possible polyphyletic origin for A. sativa from the AACC progenitor tetraploids or an origin from a progenitor of the AACC tetraploids. The sequences of the A genome group were not as clearly divided into subgroups. Although a group of sequences from the accession 'SunII' and a sequence from line Pg3, are clearly different from the others, the A genome diploid sequences were interspersed with tetraploid and hexaploid sequences.
从15个燕麦属物种（6个二倍体、6个四倍体和3个六倍体）的23份材料中扩增了燕麦（ Avena sativa L.）类受体激酶（ALrk10）基因胞外（EXC）结构域的DNA序列。引物是根据一个部分燕麦ALrk10克隆设计的，该克隆已用于将六倍体燕麦中的该基因定位到与小麦族1号和3号染色体同线的连锁群上。聚类（系统发育）分析表明，用这些引物扩增的所有燕麦DNA序列与位于小麦族物种1号染色体上的小麦和大麦序列是直系同源的。使用这些引物未扩增出小麦族3号染色体的Lrk10序列。聚类分析提供了一个位点存在多个拷贝的证据。该分析将ALrk EXC序列分为两组，其中一组包括AA和AABB基因组物种，另一组包括CC、AACC和CCCC基因组物种。在六倍体AACCDD基因组物种中发现了这两组序列，但并非在所有材料中都有。C基因组组又分为3个亚组：（i）CC二倍体和多年生同源四倍体大燕麦（Avena macrostachya）（这支持了该同源四倍体物种中存在C基因组的其他证据）；（ii）来自摩洛哥燕麦（Avena maroccana）和墨菲燕麦（Avena murphyi）的一个序列以及来自不同普通燕麦材料的几个序列；（iii）墨菲燕麦以及来自普通燕麦和不育燕麦（Avena sterilis）的序列。这表明普通燕麦可能起源于AACC祖先四倍体的多系起源，或者起源于AACC四倍体的一个祖先。A基因组组的序列没有那么明显地分为亚组。尽管来自‘SunII’材料的一组序列和Pg3品系的一个序列明显不同于其他序列，但A基因组二倍体序列与四倍体和六倍体序列相互穿插。
