Triticeae Research Institute, Sichuan Agricultural University, Chengdu, People's Republic of China.
Collaborative Innovation Center of Tissue Repair Material of Sichuan Province, China West Normal University, Nanchong, People's Republic of China.
PLoS One. 2018 Nov 8;13(11):e0200047. doi: 10.1371/journal.pone.0200047. eCollection 2018.
The phylogenetic relationships among 76 Avena taxa, representing 14 diploids, eight tetraploids, and four hexaploids were investigated by using the nuclear plastid 3-phosphoglycerate kinase gene (Pgk1). A significant deletion (131 bp) was detected in all the C genome homoeologues which reconfirmed a major structural divergence between the A and C genomes. Phylogenetic analysis indicated the Cp genome is more closely related to the polyploid species than is the Cv genome. Two haplotypes of Pgk1 gene were obtained from most of the AB genome tetraploids. Both types of the barbata group showed a close relationship with the As genome diploid species, supporting the hypothesis that both the A and B genomes are derived from an As genome. Two haplotypes were also detected in A. agadiriana, which showed close relationships with the As genome diploid and the Ac genome diploid, respectively, emphasizing the important role of the Ac genome in the evolution of A. agadiriana. Three homoeologues of the Pgk1 gene were detected in five hexaploid accessions. The homoeologues that might represent the D genome were tightly clustered with the tetraploids A. maroccana and A. murphyi, but did not show a close relationship with any extant diploid species.
利用核质 3-磷酸甘油激酶基因（Pgk1）研究了 76 个野燕麦种的系统发育关系，代表了 14 个二倍体、8 个四倍体和 4 个六倍体。在所有 C 基因组同系物中检测到一个显著的缺失（131bp），这再次证实了 A 和 C 基因组之间的主要结构分化。系统发育分析表明，Cp 基因组与多倍体物种的关系比 Cv 基因组更为密切。从大多数 AB 基因组四倍体中获得了 Pgk1 基因的两种单倍型。巴巴塔组的两种类型都与 As 基因组二倍体种密切相关，支持了 A 和 B 基因组都来自 As 基因组的假说。在 A. agadiriana 中也检测到了两种单倍型，分别与 As 基因组二倍体和 Ac 基因组二倍体密切相关，强调了 Ac 基因组在 A. agadiriana 进化中的重要作用。在五个六倍体品系中检测到了 Pgk1 基因的三个同源基因。可能代表 D 基因组的同源基因与四倍体 A. maroccana 和 A. murphyi 紧密聚类，但与任何现存的二倍体物种都没有密切关系。
