Roch-Ramel F, Diezi-Chomety F, De Rougemont D, Tellier M, Widmer J, Peters G
Am J Physiol. 1976 Mar;230(3):768-76. doi: 10.1152/ajplegacy.1976.230.3.768.
Free-flow micropuncture experiments were done in rats of three strains infused with small amounts of urate [plasma urate (P urate) = 95 +/- 8 muM]. Urate concentrations in tubular fluid were measured by an accurate chemical fluorometric ultramicromethod. In fluid from surface glomeruli, the glomerular fluid-to-plasma urate ratio [GF/P) urate] was 0.99 +/- 0.03 (n=11), i.e., lower than expected for total ultrafiltrability of plasma urate. Along proximal convolutions, net reabsorption of 55% of filtered urate was demonstrated. Small amounts of urate may have been reabsorbed between late proximal and early distal sites. Net transepithelial movements of urate did not occur in distal tubules or collecting ducts. In microperfusion experiments on proximal tubules, both a reabsorptive flow of urate (loss of perfused [2-14C]urate) and a secretory flow (entrance of cold urate into perfusate) of the same order of magnitude were demonstrated. Neither flow was influenced by simultaneous water movements. Microperfusion of Henle's loops indicated a significant but very small net reabsorption.
对三种品系的大鼠进行了自由流动微穿刺实验，这些大鼠输注了少量尿酸盐[血浆尿酸盐（P尿酸盐）= 95±8μM]。通过精确的化学荧光超微量法测量肾小管液中的尿酸盐浓度。在浅表肾小球的液体中，肾小球液与血浆尿酸盐的比率[GF/P）尿酸盐]为0.99±0.03（n = 11），即低于血浆尿酸盐完全超滤的预期值。在近端曲管中，证实了55%的滤过尿酸盐的净重吸收。少量尿酸盐可能在近端晚期和远端早期部位之间被重吸收。尿酸盐在远端小管或集合管中未发生净跨上皮移动。在近端小管的微灌注实验中，证明了尿酸盐的重吸收流（灌注的[2-14C]尿酸盐的损失）和分泌流（冷尿酸盐进入灌注液）的量级相同。两种流均不受同时发生的水移动的影响。髓袢的微灌注表明有显著但非常小的净重吸收。
