在水性聚合物双相系统中通过逆流分配法对粘多糖进行分级分离。
Fractionation of mucopolysaccharides by countercurrent distribution in aqueous polymer two-phase systems.
作者信息
Pritchard D G, Halpern R M, Halpern J A, Halpern B C, Smith R A
出版信息
Biochim Biophys Acta. 1975 Oct 9;404(2):288-99.
A new procedure for the fractionation of mucopolysaccharides based upon differences in their partition behavior in aqueous polymer two-phase systems has been devised. Systems containing dextran, poly(ethylene glycol), trimethylamino-poly(ethylene glycol), potassium bromide and sodium phosphate buffer were employed. Countercurrent distributions were performed with a miniature countercurrent distribution device designed especially for use with aqueous polymer two-phase systems. An advantage over the widely used procedures involving precipitation of mucopolysaccharides as their quaternary ammonium detergent complexes is that the countercurrent distribution pattern of a particular mucopolysaccharide is not affected by the simultaneous presence of other mucopolysaccharides. Preliminary distributions of labelled mucopolysaccharides isolated from the cells and culture medium of monolayer cultures of rat tumor cells demonstrate that the procedure is particularly well suited for the fractionation of very minute quantities of mucopolysaccharides.
基于粘多糖在水性聚合物双相系统中分配行为的差异，设计了一种新的粘多糖分级分离方法。使用了含有葡聚糖、聚乙二醇、三甲基氨基聚乙二醇、溴化钾和磷酸钠缓冲液的系统。采用专门为水性聚合物双相系统设计的微型逆流分配装置进行逆流分配。与广泛使用的将粘多糖沉淀为其季铵洗涤剂复合物的方法相比，一个优点是特定粘多糖的逆流分配模式不受其他粘多糖同时存在的影响。从大鼠肿瘤细胞单层培养物的细胞和培养基中分离出的标记粘多糖的初步分配表明，该方法特别适用于极微量粘多糖的分级分离。
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