Cooper Chris M, Davies Noel W, Menary Robert C
School of Agricultural Science and Central Science Laboratory, University of Tasmania, GPO Box 252-54, Hobart, Tasmania, Australia.
J Agric Food Chem. 2003 Apr 9;51(8):2384-9. doi: 10.1021/jf026007c.
Five C-27 apocarotenoids were detected in acetone extracts of the flowers of Boronia megastigma (Nees) using HPLC with UV-vis photodiode array and MS detection. Comparison of methylated and unmethylated extracts aided identification when considered with the UV-vis and MS data. The five apocarotenoids identified here were hydroxy-apo-10'-carotenoic acid (1), methyl hydroxy-apo-10'-carotenoate (2), apo-10'-carotenoic acid (3), apo-10'-carotenal (4), and methyl apo-10'-carotenoate (5). The data obtained was not sufficient to allow the specific isomeric forms to be unequivocally identified. The results further support speculation that the C-13 norisoprenoids found in boronia are derived from C-40 carotenoids. Possible parent molecules of beta-ionone, an important component of boronia extract, were identified. An understanding of C-13 norisoprenoid biosynthesis may assist in the selection and postharvest processing of boronia flowers for flavor and fragrance applications.
采用配有紫外 - 可见光电二极管阵列和质谱检测的高效液相色谱法，在大花博落回（Nees）花朵的丙酮提取物中检测到了5种C - 27类胡萝卜素氧化产物。将甲基化提取物和未甲基化提取物进行比较，并结合紫外 - 可见光谱和质谱数据，有助于鉴定。这里鉴定出的5种类胡萝卜素氧化产物分别是羟基 - 脱辅基 - 10'- 胡萝卜酸（1）、甲基羟基 - 脱辅基 - 10'- 胡萝卜酸甲酯（2）、脱辅基 - 10'- 胡萝卜酸（3）、脱辅基 - 10'- 胡萝卜醛（4）和脱辅基 - 10'- 胡萝卜酸甲酯（5）。所获得的数据不足以明确鉴定出具体的异构体形式。该结果进一步支持了这样的推测，即大花博落回中发现的C - 13去甲异戊二烯类化合物源自C - 40类胡萝卜素。确定了大花博落回提取物的重要成分β - 紫罗兰酮可能的母体分子。对C - 13去甲异戊二烯类化合物生物合成的了解，可能有助于大花博落回花朵在风味和香料应用方面的选择和采后加工。
