Du Hui, Disney Matthew D, Miller Benjamin L, Krauss Todd D
Department of Chemistry, and The Center for Future Health, University of Rochester, Rochester, New York 14627, USA.
J Am Chem Soc. 2003 Apr 9;125(14):4012-3. doi: 10.1021/ja0290781.
There is a keen interest in developing techniques for rapid genetic analysis that do not require labeling of an analyte. Here we demonstrate that fluorophore-tagged DNA hairpins attached to gold films can function as immobilized "molecular beacons". Two DNA hairpins incorporating portions of the Staphlococcus aureus FemA and mecR methicillin-resistance genes were attached to a gold substrate. Upon exposure to the complement, a approximately 26-fold increase in fluorescence intensity was measured corresponding to a 96 +/- 5% quenching efficiency. Studies with nonspecific DNA indicate that DNA hairpins immobilized on a gold surface retain their ability to bind complementary DNA sequences selectively.
人们对开发无需标记分析物的快速基因分析技术有着浓厚的兴趣。在此，我们证明附着在金膜上的荧光团标记的DNA发夹可以作为固定化的“分子信标”发挥作用。将包含金黄色葡萄球菌FemA和mecR耐甲氧西林基因部分的两个DNA发夹附着到金基底上。暴露于互补物后，测得荧光强度增加了约26倍，对应淬灭效率为96±5%。对非特异性DNA的研究表明，固定在金表面的DNA发夹保留了选择性结合互补DNA序列的能力。
