• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于诊断宫颈癌的沸石-氧化铁集成叉指电极传感器

Zeolite-iron oxide integrated interdigitated electrode sensor for diagnosing cervical cancer.

作者信息

Li Ling, Gopinath Subash C B, Lakshmipriya Thangavel, Subramaniam Sreeramanan, Anbu Periasamy

机构信息

Obstetrics and Gynecology, Xi'an Forth Hospital, Xi'an, 710004, China.

Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia.

出版信息

Heliyon. 2024 May 23;10(11):e31851. doi: 10.1016/j.heliyon.2024.e31851. eCollection 2024 Jun 15.

DOI:10.1016/j.heliyon.2024.e31851
PMID:38845893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11154609/
Abstract

Cervical cancer is caused by changes in the cervix that lead to precancerous cells and eventually progress to cancer. Human papillomavirus (HPV) infections are the primary cause of cervical cancer. Early detection of HPV is crucial in preventing cervical cancer, and regular screening for HPV infection can identify cell changes before they develop into cancer. While Pap smear tests are reliable for cervical cancer screening, they are critical, expensive, and labor-intensive. Therefore, researchers are focusing on identifying blood-based biomarkers using biosensors for cervical cancer screening. HPV strains 16, 45, and 18 are common culprits in cervical cancer. This study aimed to develop an HPV-16 DNA biosensor on a zeolite-iron oxide (zeolite-IO) modified interdigitated electrode (IDE) sensor. The DNA probe was immobilized on the IDE through amine-modified zeolite-IO, enhancing the hybridization of the target and DNA probe. The detection limit of the DNA-DNA duplex was found to be 7.5 pM with an R value of 0.9868. Additionally, control experiments with single and triple mismatched sequences showed no increase in current responses, and the identification of target DNA in a serum-spiked sample indicated specific and selective target identification.

摘要

宫颈癌是由宫颈的变化引起的，这些变化会导致癌前细胞，最终发展成癌症。人乳头瘤病毒（HPV）感染是宫颈癌的主要原因。早期检测HPV对于预防宫颈癌至关重要，定期筛查HPV感染可以在细胞变化发展成癌症之前识别出来。虽然巴氏涂片检查对宫颈癌筛查可靠，但它们至关重要、成本高昂且劳动强度大。因此，研究人员正专注于使用生物传感器识别基于血液的生物标志物用于宫颈癌筛查。HPV 16型、45型和18型毒株是宫颈癌的常见元凶。本研究旨在在沸石 - 氧化铁（zeolite-IO）修饰的叉指电极（IDE）传感器上开发一种HPV-16 DNA生物传感器。DNA探针通过胺修饰的zeolite-IO固定在IDE上，增强了靶标与DNA探针的杂交。发现DNA-DNA双链体的检测限为7.5 pM，R值为0.9868。此外，对单链和三链错配序列的对照实验表明电流响应没有增加，并且在加样血清样本中对靶标DNA的识别表明了特异性和选择性的靶标识别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/025dedd51674/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/be86118b6090/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/aec6a1c3b128/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/3b51ec149d75/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/db14b2896239/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/eb596b730a67/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/025dedd51674/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/be86118b6090/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/aec6a1c3b128/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/3b51ec149d75/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/db14b2896239/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/eb596b730a67/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d9/11154609/025dedd51674/gr6.jpg

相似文献

1
Zeolite-iron oxide integrated interdigitated electrode sensor for diagnosing cervical cancer.用于诊断宫颈癌的沸石-氧化铁集成叉指电极传感器
Heliyon. 2024 May 23;10(11):e31851. doi: 10.1016/j.heliyon.2024.e31851. eCollection 2024 Jun 15.
2
Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy.人乳头瘤病毒 DNA 生物标志物分析在宫颈癌中的应用：金纳米颗粒偶联四价链霉亲和素-生物素策略的信号增强。
Int J Biol Macromol. 2019 Aug 1;134:354-360. doi: 10.1016/j.ijbiomac.2019.05.044. Epub 2019 May 10.
3
Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles.利用二氧化钛纳米粒子修饰的叉指电极对人乳头瘤病毒 16 型 DNA 的伏安法测定。
Mikrochim Acta. 2019 May 8;186(6):336. doi: 10.1007/s00604-019-3445-2.
4
Target DNA detection of human papilloma virus-16 E7 gene by capture-target-reporter sandwich on interdigitated electrode sensor.采用交错式电极传感器的捕获-靶标-报告分子三明治夹心法检测人乳头瘤病毒 16 型 E7 基因的靶 DNA
Int J Biol Macromol. 2019 Dec 1;141:564-569. doi: 10.1016/j.ijbiomac.2019.09.012. Epub 2019 Sep 4.
5
Fly Ash-Based Zeolite-Complexed Polyethylene-Glycol on an Interdigitated Electrode Surface for High-Performance Determination of Diabetes Mellitus.基于粉煤灰的沸石-复合聚乙二醇在叉指电极表面用于高性能糖尿病检测。
Int J Nanomedicine. 2020 Sep 8;15:6619-6629. doi: 10.2147/IJN.S264645. eCollection 2020.
6
Zeolite-iron oxide nanocomposite from fly ash formed a 'clubbell' structure: integration of cardiac biocapture macromolecules in serum on microelectrodes.粉煤灰合成沸石-氧化亚铁纳米复合材料形成“哑铃”结构：将心脏生物捕获大分子整合到微电极上的血清中。
Mikrochim Acta. 2021 May 15;188(6):187. doi: 10.1007/s00604-021-04834-w.
7
Determination of the diagnostic accuracy of testing for high-risk (HR) human papillomavirus (HPV) types 16, 18 and 45 in precancerous cervical lesions: preliminary data.检测高危型（HR）人乳头瘤病毒（HPV）16、18 和 45 型在癌前宫颈病变中的诊断准确性的测定：初步数据。
J Clin Virol. 2009 Nov;46 Suppl 3:S11-5. doi: 10.1016/S1386-6532(09)70295-1.
8
Detection and genotyping of HPV-DNA through different types of diagnostic platforms in liquid-based cervical-cytology samples.通过不同类型诊断平台对液基宫颈细胞学样本中的人乳头瘤病毒DNA进行检测和基因分型。
Pathologica. 2018 Dec;110(4):294-301.
9
Electrochemical detection of human papillomavirus DNA type 16 using a pyrrolidinyl peptide nucleic acid probe immobilized on screen-printed carbon electrodes.基于固定在丝网印刷碳电极上的吡咯烷二肽核酸探针电化学检测人乳头瘤病毒 16 型 DNA。
Biosens Bioelectron. 2014 Apr 15;54:428-34. doi: 10.1016/j.bios.2013.11.023. Epub 2013 Nov 20.
10
Diagnostic Performance of HPV E6/E7 mRNA and HPV DNA Assays for the Detection and Screening of Oncogenic Human Papillomavirus Infection among Woman with Cervical Lesions in China.人乳头瘤病毒E6/E7信使核糖核酸和人乳头瘤病毒脱氧核糖核酸检测法在中国宫颈病变女性中检测和筛查致癌性人乳头瘤病毒感染的诊断性能
Asian Pac J Cancer Prev. 2015;16(17):7633-40. doi: 10.7314/apjcp.2015.16.17.7633.

本文引用的文献

1
Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16.电化学电阻式 DNA 生物传感器用于 HPV 16 型的检测。
Molecules. 2021 Jun 5;26(11):3436. doi: 10.3390/molecules26113436.
2
Zeolite-iron oxide nanocomposite from fly ash formed a 'clubbell' structure: integration of cardiac biocapture macromolecules in serum on microelectrodes.粉煤灰合成沸石-氧化亚铁纳米复合材料形成“哑铃”结构：将心脏生物捕获大分子整合到微电极上的血清中。
Mikrochim Acta. 2021 May 15;188(6):187. doi: 10.1007/s00604-021-04834-w.
3
The application of DNA polymerases and Cas9 as representative of DNA-modifying enzymes group in DNA sensor design (review).
DNA 聚合酶和 Cas9 作为 DNA 修饰酶类的代表在 DNA 传感器设计中的应用（综述）。
Biosens Bioelectron. 2021 Mar 1;175:112867. doi: 10.1016/j.bios.2020.112867. Epub 2020 Dec 3.
4
The Recent Development of Hybridization Chain Reaction Strategies in Biosensors.杂交链式反应策略在生物传感器中的最新发展。
ACS Sens. 2020 Oct 23;5(10):2977-3000. doi: 10.1021/acssensors.0c01453. Epub 2020 Oct 1.
5
Ultrasensitive DNA biosensor for hepatitis B virus detection based on tin-doped WO/InO heterojunction nanowire photoelectrode under laser amplification.基于激光放大的掺锡 WO/InO 异质结纳米线光电阴极的乙型肝炎病毒超灵敏 DNA 生物传感器
Anal Bioanal Chem. 2020 Sep;412(22):5367-5377. doi: 10.1007/s00216-020-02752-z. Epub 2020 Jun 13.
6
DNA Origami as Emerging Technology for the Engineering of Fluorescent and Plasmonic-Based Biosensors.DNA折纸术：用于构建基于荧光和等离子体的生物传感器的新兴技术
Materials (Basel). 2020 May 9;13(9):2185. doi: 10.3390/ma13092185.
7
Global burden of cancer attributable to infections in 2018: a worldwide incidence analysis.2018 年归因于感染的癌症全球负担：全球发病率分析。
Lancet Glob Health. 2020 Feb;8(2):e180-e190. doi: 10.1016/S2214-109X(19)30488-7. Epub 2019 Dec 17.
8
A fluorescence Nano-biosensors immobilization on Iron (MNPs) and gold (AuNPs) nanoparticles for detection of Shigella spp.基于铁（MNPs）和金（AuNPs）纳米粒子的荧光纳米生物传感器固定化用于志贺氏菌属的检测
Mater Sci Eng C Mater Biol Appl. 2019 Dec;105:110113. doi: 10.1016/j.msec.2019.110113. Epub 2019 Aug 22.
9
Target DNA detection of human papilloma virus-16 E7 gene by capture-target-reporter sandwich on interdigitated electrode sensor.采用交错式电极传感器的捕获-靶标-报告分子三明治夹心法检测人乳头瘤病毒 16 型 E7 基因的靶 DNA
Int J Biol Macromol. 2019 Dec 1;141:564-569. doi: 10.1016/j.ijbiomac.2019.09.012. Epub 2019 Sep 4.
10
Squamous Cell Carcinoma Biomarker Sensing on a Strontium Oxide-Modified Interdigitated Electrode Surface for the Diagnosis of Cervical Cancer.基于氧化锶修饰叉指电极表面的鳞状细胞癌生物标志物传感用于宫颈癌诊断。
Biomed Res Int. 2019 Apr 7;2019:2807123. doi: 10.1155/2019/2807123. eCollection 2019.