通过抑制核磁共振波谱中极化转移的J耦合实现定量二维异核单量子相干谱（Q-HSQC）：在木材木质素中的应用

Quantitative 2D HSQC (Q-HSQC) via suppression of J-dependence of polarization transfer in NMR spectroscopy: application to wood lignin.

作者信息

Heikkinen Sami, Toikka Merja M, Karhunen Pirkko T, Kilpeläinen Ilkka A

机构信息

NMR laboratory, Structural Biology and Biophysics, Institute of Biotechnology, P.O. Box 65, FIN-00014 University of Helsinki, Finland.

出版信息

J Am Chem Soc. 2003 Apr 9;125(14):4362-7. doi: 10.1021/ja029035k.

DOI:10.1021/ja029035k

PMID:12670260

Abstract

A quantitative method to record (1)H-(13)C correlation NMR spectra (Q-HSQC) is presented. The suppression of (1)J(CH)-dependence is achieved by modulating the polarization transfer delays of HSQC. In addition, the effect of homonuclear couplings, as well as relaxation during the pulse sequence are discussed. We developed the Q-HSQC approach for the quantitative analysis of wood lignin, a complex polymer where it has been difficult to obtain reliable data on the relative amounts of different structural units. The current method is applicable to a variety of complex mixtures, where normal 1D (1)H- and (13)C-NMR methods fail.

摘要

本文提出了一种记录氢-碳相关核磁共振谱（Q-HSQC）的定量方法。通过调制HSQC的极化转移延迟实现了对1J(CH)依赖性的抑制。此外，还讨论了同核耦合的影响以及脉冲序列期间的弛豫。我们开发了Q-HSQC方法用于木材木质素的定量分析，木质素是一种复杂的聚合物，很难获得不同结构单元相对含量的可靠数据。当前方法适用于各种复杂混合物，而常规的一维氢谱和碳谱核磁共振方法在此失效。