Romanian Academy P. Poni Institute of Macromolecular Chemistry, Department of Physical Chemistry of Polymers, 41A Gr. Ghica Voda Alley, Ro.700487, Iasi, Romania.
Appl Spectrosc. 2010 Sep;64(9):1054-60. doi: 10.1366/000370210792434413.
X-ray diffraction, scanning electron microscopy (SEM), and solid-state cross-polarization magic-angle-spinning (CP/MAS) (13)C-NMR spectroscopy were applied to determine changes over time in the morphology and crystallinity of lime wood (Tilia cordata Miller) generated by the soft-rot fungi. Wood samples were inoculated with Trichoderma viride Pers for various durations up to 84 days. Structural and morphological modifications were assessed by comparing the structural features of decayed lime wood samples with references. Significant morphology changes such as defibration or small cavities were clearly observed on the SEM micrographs of lime wood samples exposed to fungi. Following the deconvolution process of the diffraction patterns, the degree of crystallinity, apparent lateral crystallite size, the proportion of crystallite interior chains, and the cellulose fraction have been determined. It was found that all crystallographic data vary with the duration of exposure to fungi. The degree of crystallinity and cellulose fraction tend to decrease, whereas the apparent lateral crystallite size and the proportion of crystallite interior chains increase with prolonged biodegradation processes. The most relevant signals in CP/MAS (13)C-NMR spectra were assigned according to literature data. The differences observed were discussed in terms of lignin and cellulose composition: by fixing the lignin reference signal intensity, the cellulose and hemicelluloses moieties showed a relative decrease compared to the lignin signals in decayed wood.
X 射线衍射、扫描电子显微镜（SEM）和固态交叉极化魔角旋转（CP/MAS）（13）C-NMR 光谱被应用于确定软腐真菌对椴木（Tilia cordata Miller）形态和结晶度随时间的变化。将木霉（Trichoderma viride Pers）接种到木材样本中，持续时间长达 84 天。通过与参考物比较腐朽椴木样本的结构特征来评估结构和形态的变化。SEM 显微照片清楚地观察到暴露于真菌的椴木样本的明显形态变化，如纤维化或小空腔。通过对衍射图谱的去卷积处理，确定了结晶度、表观侧微晶尺寸、晶体内链比例和纤维素分数。结果发现，所有结晶数据都随暴露于真菌的时间而变化。结晶度和纤维素分数趋于降低，而表观侧微晶尺寸和晶体内链比例随着生物降解过程的延长而增加。根据文献数据对 CP/MAS（13）C-NMR 光谱中的相关信号进行了分配。根据木质素和纤维素组成讨论了观察到的差异：通过固定木质素参考信号强度，与腐朽木材中的木质素信号相比，纤维素和半纤维素部分相对减少。
