John Luo Jiin-Chyuan, Cheng Tsun-Jen, Du Chung-Li, Wang Jung-Der
Department of Public Health, Chang Gung Medical College, 259 Wen-Hua 1st Road, Kwei-Shan, Tao-Yuan, Taiwan ROC.
Cancer Detect Prev. 2003;27(2):94-101. doi: 10.1016/s0361-090x(03)00021-7.
To determine the presence of Asp13-p21-ki-ras oncoprotein and p53 oncoprotein in the plasma of vinyl chloride monomer (VCM)-workers in Taiwan.
We used enhanced chemiluminescence (ECL) western blotting to detect Asp13-p21-ki-ras and ELISA to detect mutant p53 protein (p53-Ag) and anti-p53 antibodies (p53-Ab) in the plasma of VCM-exposed workers.
Twenty-five out of 251 (10%) VCM-workers were positive for Asp13-p21-ki-ras in plasma, but 0 out of 36 controls were positive. There were 15 out of 95 (15.8%) plasma-positives among the more highly exposed (> 480 ppm-month) workers and 10 out of 156 (6.4%) plasma-positives among the lesser exposed (< or = 480 ppm-month). Compared to the unexposed controls, age and drinking adjusted odds ratios (95% CI) were 1.2 (0.1, 9.8) in the lower exposed workers, and 4.8 (0.8, 28) in the higher exposed workers, and there was a significant linear trend between exposure and plasma positivity (P=0.001). Thirty-three out of 251 (13.2%) VCM-workers were positive for the p53 over-expression (10% with positive p53-Ag and 2.8% with positive p53-Ab). There was a significant association between cumulative VCM exposure concentration and positive p53 expression (P=0.032) among VCM-workers after adjusting for age, hepatitis, drinking and smoking status.
Asp13-p21-ki-ras oncoprotein and p53 over-expression (p53-Ag or p53-Ab) can be found in the plasma of VCM-workers in Taiwan, and a significant dose-response relationship exists between plasma oncoproteins expression and VCM exposure.
确定台湾氯乙烯单体（VCM）作业工人血浆中Asp13-p21-ki-ras癌蛋白和p53癌蛋白的存在情况。
我们采用增强化学发光（ECL）免疫印迹法检测VCM接触工人血浆中的Asp13-p21-ki-ras，并采用酶联免疫吸附测定（ELISA）法检测突变型p53蛋白（p53-Ag）和抗p53抗体（p53-Ab）。
251名VCM作业工人中有25名（10%）血浆Asp13-p21-ki-ras呈阳性，而36名对照者中无一人阳性。在接触程度较高（>480 ppm-月）的工人中，95名中有15名（15.8%）血浆呈阳性；在接触程度较低（≤480 ppm-月）的工人中，156名中有10名（6.4%）血浆呈阳性。与未接触的对照者相比，年龄和饮酒情况调整后的优势比（95%可信区间）在接触程度较低的工人中为1.2（0.1，9.8），在接触程度较高的工人中为4.8（0.8，28），且接触与血浆阳性之间存在显著的线性趋势（P=0.001）。251名VCM作业工人中有33名（13.2%）p53过度表达呈阳性（10%为p53-Ag阳性，2.8%为p53-Ab阳性）。在调整年龄、肝炎、饮酒和吸烟状况后，VCM作业工人中累积VCM接触浓度与p53阳性表达之间存在显著关联（P=0.032）。
台湾VCM作业工人血浆中可检测到Asp13-p21-ki-ras癌蛋白和p53过度表达（p53-Ag或p53-Ab），血浆癌蛋白表达与VCM接触之间存在显著的剂量反应关系。
