利用锌卟啉的可见光敏化作用从多糖仿生制氢

Bio-mimetic hydrogen production from polysaccharide using the visible light sensitization of zinc porphyrin.

作者信息

Saiki Yoshinobu, Amao Yutaka

机构信息

Department of Applied Chemistry, Oita University, Dannoharu 700, Oita 870-1192, Japan.

出版信息

Biotechnol Bioeng. 2003 Jun 20;82(6):710-4. doi: 10.1002/bit.10622.

DOI:10.1002/bit.10622

PMID:12673771

Abstract

A biohydrogen production system coupling the polysaccharide such as sucrose and maltose degradation with invertase and glucose dehydrognase (GDH) and hydrogen production with colloidal platinum as hydrogen-evolved catalyst using the visible light-induced photosensitization of water-soluble zinc porphyrin, zinc tetraphenylporphyrin tetrasulfonate (ZnTPPS) has been investigated. Continuous hydrogen gas production was observed when the sample solution containing polysaccharide, invertase, GDH, nicotinamide adenine dinucreotide (NAD(+)), ZnTPPS, methylviologen (an electron relay reagent), and colloidal platinum was irradiated by visible light. After 240-min irradiation, the amount of hydrogen production in the system using sucrose and maltose was estimated to be 3.1 and 0.35 micromol, respectively.

摘要

研究了一种生物制氢系统，该系统利用水溶性锌卟啉四苯基卟啉四磺酸锌（ZnTPPS）的可见光诱导光敏化作用，将蔗糖和麦芽糖等多糖的降解与转化酶和葡萄糖脱氢酶（GDH）相耦合，并以胶体铂作为析氢催化剂进行产氢。当含有多糖、转化酶、GDH、烟酰胺腺嘌呤二核苷酸（NAD(+)）、ZnTPPS、甲基紫精（一种电子中继试剂）和胶体铂的样品溶液受到可见光照射时，观察到了持续的氢气产生。经过240分钟的照射后，使用蔗糖和麦芽糖的系统中产氢量分别估计为3.1微摩尔和0.35微摩尔。