利用锌卟啉的可见光敏化作用通过乳酸脱氢酶生产乳酸。

Lactic acid production with lactate dehydrogenase using the visible light sensitization of zinc porphyrin.

作者信息

Miyatani Rie, Amao Yutaka

机构信息

Department of Applied Chemistry, Oita University, Dannoharu 700, Oita 870-1192, Japan.

出版信息

Photochem Photobiol Sci. 2004 Jul;3(7):681-3. doi: 10.1039/b309596g. Epub 2004 May 20.

DOI:10.1039/b309596g

PMID:15239004

Abstract

Lactic acid production with L-lactate dehydrogenase from Pig heart (LDH) and reduced methyl viologen produced by the visible light photosensitization of zinc tetrakis(4-methylpyridyl)porphyrin (ZnTMPyP) was investigated. When the sample solution containing triethanolamine as an electron-donating reagent, ZnTMPyP, methyl viologen as an electron carrier, pyruvic acid and LDH in potassium phosphate buffer (pH 7) was irradiated, lactic acid production was observed. After 240 min irradiation, the amount of lactic acid production and the yield of pyruvic acid to lactic acid were estimated to be 0.17 mmol dm(-3) and 17.0%, respectively.

摘要

研究了利用猪心L-乳酸脱氢酶（LDH）和通过四（4-甲基吡啶基）锌卟啉（ZnTMPyP）的可见光光敏化产生的还原甲基紫精来生产乳酸的情况。当含有作为供电子试剂的三乙醇胺、ZnTMPyP、作为电子载体的甲基紫精、丙酮酸和LDH的样品溶液在磷酸钾缓冲液（pH 7）中受到照射时，观察到了乳酸的产生。照射240分钟后，乳酸的产生量和丙酮酸向乳酸的转化率分别估计为0.17 mmol dm(-3)和17.0%。