来自墨西哥的一株霍乱弧菌O139临床分离株的特性分析。

Characterization of a clinical Vibrio cholerae O139 isolate from Mexico.

作者信息

Parveen Salina, Farrah Samuel R, Gonzalez-Bonilla Celia, Zamudio Altagracia V, Tamplin Mark L

机构信息

Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.

出版信息

Can J Microbiol. 2003 Jan;49(1):65-70. doi: 10.1139/w03-004.

DOI:10.1139/w03-004

PMID:12674350

Abstract

Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenotypic characteristics of this strain were identical to those of V. cholerae O1 biotype El Tor. Unlike ctxA-positive V. cholerae O139 strains, this strain was sensitive to a wide panel of antibiotics, including ampicillin, chloramphenicol, ciprofloxacin, gentamicin, furazolidone, nalidixic acid, nitrofurantoin, tetracycline, trimethoprim-sulfamethoxazole, and streptomycin, but was resistant to polymyxin B. Ribotype and pulsed-field gel electrophoresis profiles of INDRE1 differed from those of ctxA-positive V. cholerae O139 and other V. cholerae strains. Phenotypic characteristics of the Mexico strain were similar to those reported for V. cholerae O139 isolates from Argentina and Sri Lanka.

摘要

霍乱弧菌O139的致病菌株拥有霍乱毒素A亚基（ctxA）基因以及毒素共调节菌毛（tcpA）基因。我们报告了从墨西哥一名被诊断患有胃肠道疾病的患者身上分离出一株ctxA阴性、tcpA阴性的霍乱弧菌O139菌株（INDREI）。该菌株的某些表型特征与霍乱弧菌O1生物型埃尔托生物型相同。与ctxA阳性的霍乱弧菌O139菌株不同，该菌株对多种抗生素敏感，包括氨苄西林、氯霉素、环丙沙星、庆大霉素、呋喃唑酮、萘啶酸、呋喃妥因、四环素、甲氧苄啶-磺胺甲恶唑和链霉素，但对多粘菌素B耐药。INDRE1的核糖体分型和脉冲场凝胶电泳图谱与ctxA阳性的霍乱弧菌O139和其他霍乱弧菌菌株不同。墨西哥菌株的表型特征与从阿根廷和斯里兰卡分离出的霍乱弧菌O139菌株的报道特征相似。