Budimir M, Plesnicar M, Kljajić R
Arch Environ Contam Toxicol. 1976;4(2):166-74. doi: 10.1007/BF02221021.
The effects of the herbicide 4(2,4-dichlorophenoxy)butyric acid (2,4-DB) and fungicide N-(trichloromethyltio)-4-cyclohexene-1,2-dicarboximide (captan) on electron transport processes of mitochondria and chloroplasts have been investigated. Chloroplasts, isolated from spinach leaves (Spinacia oleracea L.), were treated with pesticide prior to the addition of electron acceptor and ADP. White potato (Solanum tuberosum L.) mitochondria were either incubated with pesticide before the addition of substrate, or they were treated with pesticide after the addition of substrate and ADP. Captan inhibited oxidation of malate by mitochondria and acted as an uncoupler. With succinate as sunstrate captan was found to stimulate state 4 respiration, as substrate captan was found to stimulate state 4 respiration, with the loss of coupled phosphorylation only at higher concentrations of fungicide. The herbicide 2,4-DB appeared to be 5 to 10 times less effective than captain. Both compounds inhibited phosphrylation-coupled succinate oxidation at higher concentrations and malate-coupled phosphorylation at lower concentrations. They acted as inhibitors of NADH-cytochrome c reductase. Both pesticides inhibited noncyclic electron transport in chloroplasts. The rate of ferricyanide reduction in the presence and absence of phosphorylating agents was reduced, and although the rate of ATP generation was reduced also, the P/2e ratio was not changed much under the influence of pesticides.
已对除草剂4-(2,4-二氯苯氧基)丁酸(2,4-DB)和杀菌剂N-(三氯甲基硫代)-4-环己烯-1,2-二甲酰亚胺(克菌丹)对线粒体和叶绿体电子传递过程的影响进行了研究。从菠菜叶(菠菜)中分离出的叶绿体，在添加电子受体和ADP之前先用农药处理。将马铃薯(马铃薯)线粒体在添加底物之前与农药一起温育，或者在添加底物和ADP之后用农药处理。克菌丹抑制线粒体对苹果酸的氧化，并起解偶联剂的作用。以琥珀酸为底物时，发现克菌丹能刺激状态4呼吸，随着杀菌剂浓度升高，仅在较高浓度的杀菌剂下才会出现偶联磷酸化的丧失。除草剂2,4-DB的效果似乎比克菌丹低5至10倍。两种化合物在较高浓度下均抑制磷酸化偶联的琥珀酸氧化，在较低浓度下抑制苹果酸偶联的磷酸化。它们作为NADH-细胞色素c还原酶的抑制剂起作用。两种农药均抑制叶绿体中的非循环电子传递。在有和没有磷酸化剂的情况下，铁氰化物的还原速率均降低，尽管ATP生成速率也降低了，但在农药影响下P/2e比值变化不大。
