Fan Jing, Chen Yahong, Feng Suling, Ye Cunling, Wang Jianji
School of Chemical & Environmental Sciences, Henan Normal University, Key Laboratory of Environmental Science and Engineering of Henan Education Commission, Xinxiang, Henan 453002, People's Republic of China.
Anal Sci. 2003 Mar;19(3):419-22. doi: 10.2116/analsci.19.419.
A rapid and sensitive flow-injection spectrophotometric method is proposed for the determination of sulfadiazine and sulfamethoxazole. This method is based on the diazotization of sulfonamide with sodium nitrite, and a coupling reaction of the diazo-compound with alpha-naphthylamine. The optimum experimental conditions are obtained by using the controlled and weighted centroid simplex method. The linear ranges for the determination of sulfadiazine and sulfamethoxazole are 0.2-20 microg ml(-1) and 0.1-20 microg ml(-1), and their detection limits are 0.06 microg ml(-1) and 0.05 microg ml(-1), respectively, and the sampling frequency is 130 samples per hour. The method has been used to determine sulfadiazine and sulfamethoxazole in pharmaceuticals and urine without separation. The results are in agreement with those obtained by a high-performance liquid chromatograph technique at the 95% confidence level.
提出了一种快速灵敏的流动注射分光光度法测定磺胺嘧啶和磺胺甲恶唑。该方法基于磺胺类药物与亚硝酸钠的重氮化反应，以及重氮化合物与α-萘胺的偶联反应。通过使用控制加权质心单纯形法获得了最佳实验条件。磺胺嘧啶和磺胺甲恶唑的测定线性范围分别为0.2 - 20μg ml⁻¹和0.1 - 20μg ml⁻¹，检测限分别为0.06μg ml⁻¹和0.05μg ml⁻¹，采样频率为每小时130个样品。该方法已用于不经分离直接测定药物和尿液中的磺胺嘧啶和磺胺甲恶唑。结果与高效液相色谱技术在95%置信水平下获得的结果一致。
