Kappe Reinhard, Rimek Dagmar
Institut für Medizinische Mikrobiologie und Hygiene am HELIOS Klinikum Erfurt, Nordhäuser Str. 74, D-99089 Erfurt, Germany.
Prog Drug Res. 2003;Spec No:39-57. doi: 10.1007/978-3-0348-7974-3_3.
In this chapter, we focus on diagnostic laboratory methods that are necessary and suitable for providing physicians with a timely and accurate diagnosis of fungal diseases. After discussing some pre-analytical aspects, the complete set of methods, i.e., microscopy, histopathology, culture, antigen detection, DNA detection, and antibody detection, is concisely described. Identification techniques depend on the fungal group involved. Therefore, separate paragraphs are dedicated to the identification of yeasts and filamentous fungi, which include moulds, dermatophytes, and dimorphic fungi.
在本章中，我们重点关注那些对于为医生及时、准确诊断真菌病而言必要且合适的诊断实验室方法。在讨论了一些分析前的方面之后，简要描述了一整套方法，即显微镜检查、组织病理学、培养、抗原检测、DNA检测和抗体检测。鉴定技术取决于所涉及的真菌类别。因此，分别用段落专门介绍酵母和丝状真菌的鉴定，丝状真菌包括霉菌、皮肤癣菌和双相真菌。
