培养的中国仓鼠卵巢细胞质膜的分离与鉴定。

The isolation and characterization of the plasma membrane of cultured chinese hamster ovary cells.

作者信息

Juliano R L, Gagalang E

出版信息

Can J Biochem. 1975 Aug;53(8):914-20. doi: 10.1139/o75-126.

Abstract

Plasma membranes were prepared from cultured Chinese hamster ovary cells utilizing a two-phase polymer system and were characterized by enzymatic and chemical assay, and by electron microscopy. The usual degree of purification of presumptive membrane markers such as Na+-K+ ATPase (ATP phosphohydrolase, EC 3.6.1.3) ranged from three-to eightfold. Gel electrophoresis in SDS revealed several polypeptides and two glycopeptides which were enriched in the plasma membrane fraction.

摘要

利用双相聚合物系统从培养的中国仓鼠卵巢细胞中制备质膜，并通过酶促和化学分析以及电子显微镜对其进行表征。推测的膜标志物如Na⁺-K⁺ ATP酶（ATP磷酸水解酶，EC 3.6.1.3）的通常纯化程度为3至8倍。SDS中的凝胶电泳显示出几种多肽和两种糖肽，它们在质膜组分中富集。