Zam Z S, Cerda J, Polack F M
Invest Ophthalmol Vis Sci. 1980 Jun;19(6):648-52.
The plasma membtanes of normal rabbit endothelail cells were isolated by the use of an aqueous two-phase polymer system. The membrane fraction was identified by electron microscopy, and a fivefold to eightfold increase in the specific activity of two plasma membrane markers. Na+-K+-ATPase and 5'nucleotidase was found. Recovery of the enzyme markers averaged 45% and 22%, respectively. Analysis of the purified membranes for glucose-6-phosphatase, a marker for endoplasmic reticulum, showed no contamination by this structure. This method for cell membrane characterization is promising in determining the enzymatic alterations of diseased corneas.
采用双水相聚合物系统分离正常兔内皮细胞的质膜。通过电子显微镜鉴定膜组分，发现两种质膜标志物（Na⁺-K⁺-ATP酶和5'-核苷酸酶）的比活性增加了五到八倍。酶标志物的回收率平均分别为45%和22%。对纯化膜进行内质网标志物葡萄糖-6-磷酸酶分析，结果表明该结构未受污染。这种细胞膜表征方法在确定患病角膜的酶学改变方面很有前景。
