膜联蛋白I在人食管鳞状细胞癌中从细胞膜向核膜的转位。
Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma.
作者信息
Liu Yu, Wang Hui-Xin, Lu Ning, Mao You-Sheng, Liu Fang, Wang Ying, Zhang Hai-Rong, Wang Kun, Wu Min, Zhao Xiao-Hang
机构信息
National Lab. of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing P. O. Box 2258, Beijing 100021, China.
出版信息
World J Gastroenterol. 2003 Apr;9(4):645-9. doi: 10.3748/wjg.v9.i4.645.
AIM
To investigate the alteration of the annexin I subcellular localization in esophageal squamous cell carcinoma (ESCC) and the correlation between the translocation and the tumorigenesis of ESCC.
METHODS
The protein localization of annexin I was detected in both human ESCC tissues and cell line via the indirect immunofluorescence strategy.
RESULTS
In the normal esophageal epithelia the annexin I was mainly located on the plasma membrane and formed a consecutive typical trammels net. Annexin I protein also expressed dispersively in cytoplasm and the nuclei without specific localization on the nuclear membrane. In esophageal cancer annexin I decreased very sharply with scattered disappearance on the cellular membrane, however it translocated and highly expressed on the nuclear membrane, which was never found in normal esophageal epithelia. In cultured esophageal cancer cell line annexin I protein was also focused on the nuclear membrane, which was consistent with the result from esophageal cancer tissues.
CONCLUSION
This observation suggests that the translocation of annexin I protein in ESCC may correlate with the tumorigenesis of the esophageal cancer.
目的
研究膜联蛋白I在食管鳞状细胞癌（ESCC）中的亚细胞定位变化，以及这种易位与ESCC肿瘤发生之间的相关性。
方法
通过间接免疫荧光法检测人ESCC组织和细胞系中膜联蛋白I的蛋白定位。
结果
在正常食管上皮中，膜联蛋白I主要位于质膜上，形成连续的典型网格状。膜联蛋白I蛋白也分散表达于细胞质和细胞核中，在核膜上无特异性定位。在食管癌中，膜联蛋白I急剧减少，细胞膜上出现散在消失，然而它易位并在核膜上高表达，这在正常食管上皮中从未发现。在培养的食管癌细胞系中，膜联蛋白I蛋白也集中在核膜上，这与食管癌组织的结果一致。
结论
该观察结果表明，ESCC中膜联蛋白I蛋白的易位可能与食管癌的肿瘤发生相关。
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