Zhang Lin-chao, Zheng Xin-min, Yang Zhi-wei, Li Shi-wen
Department of Urology, Zhongnan Hospital, Wuhan University, Hubei, China.
Zhonghua Nan Ke Xue. 2006 Apr;12(4):352-4.
To investigate the relationship between germ cell apoptosis and expressions of Bcl-2 and Bax in contralateral testes of experimental unilateral cryptorchid rats.
Twenty male Sprague-Dawley rats were randomly divided into 2 groups (namely cryptorchid group and control group), with 10 rats in each group. Cryptorchid animal model was established, and contralateral testes were captured 90 days later. The evidence of germ cell apoptosis was detected by TUNEL assay. The expressions of Bcl-2 and Bax were detected by immunohistochemical method.
In the contralateral testes of experimental unilateral cryptorchidism, apoptosis index of germ cell and Bax expression significantly increased compared with those in the control group, respectively (P < 0.01), while Bcl-2 expression and testis weight obviously decreased (P <0.01). The apoptotic cells were mostly pachytene spermatocytes and round spermatids.
The germ cell apoptosis is highly correlated with expressions of Bcl-2 and Bax in contralateral testes of experimental unilateral cryptorchidism. Bcl-2/Bax plays an important role in germ cell apoptosis.
探讨实验性单侧隐睾大鼠对侧睾丸生殖细胞凋亡与Bcl-2和Bax表达之间的关系。
将20只雄性Sprague-Dawley大鼠随机分为2组（即隐睾组和对照组），每组10只。建立隐睾动物模型，90天后摘取对侧睾丸。采用TUNEL法检测生殖细胞凋亡情况。采用免疫组织化学方法检测Bcl-2和Bax的表达。
实验性单侧隐睾大鼠对侧睾丸中，生殖细胞凋亡指数和Bax表达分别较对照组显著升高（P<0.01），而Bcl-2表达和睾丸重量明显降低（P<0.01）。凋亡细胞主要是粗线期精母细胞和圆形精子细胞。
实验性单侧隐睾大鼠对侧睾丸生殖细胞凋亡与Bcl-2和Bax表达高度相关。Bcl-2/Bax在生殖细胞凋亡中起重要作用。
