Cai B, Han Y, Liu B, Ren Y, Jiang S
College of Life Sciences, Naikai University, Tianjin, China.
Lett Appl Microbiol. 2003;36(5):272-6. doi: 10.1046/j.1472-765x.2003.01307.x.
To isolate and characterize atrazine-degrading bacteria in order to identify suitable candidates for potential use in bioremediation of atrazine contamination.
A high efficiency atrazine-degrading bacterium, strain AD1, which was capable of utilizing atrazine as a sole nitrogen source for growth, was isolated from industrial wastewater. 16S rDNA sequencing identified AD1 as an Arthrobacter sp. The atrazine chlorohydrolase gene (atzA) isolated from strain AD1 differed from that found in the Pseudomonas sp. ADP by only one nucleotide. However, it was found located on the bacterial chromosome rather than on plasmids as previously reported for other bacteria.
Atrazine chlorohydrolase gene, atzA, either encoded by chromosome or plasmid, is highly conserved.
Comparison analysis of atrazine degradation gene structure and arrangement in this and other bacteria provides insight into our understanding of the ecology and evolution of atrazine-degrading bacteria.
分离并鉴定阿特拉津降解菌，以便确定可用于生物修复阿特拉津污染的合适候选菌株。
从工业废水中分离出一株高效阿特拉津降解菌AD1，该菌株能够利用阿特拉津作为唯一氮源进行生长。16S rDNA测序鉴定AD1为节杆菌属（Arthrobacter sp.）。从菌株AD1中分离出的阿特拉津氯水解酶基因（atzA）与假单胞菌属（Pseudomonas sp.）ADP中的该基因仅相差一个核苷酸。然而，发现它位于细菌染色体上，而非如先前报道的其他细菌那样位于质粒上。
无论是由染色体还是质粒编码的阿特拉津氯水解酶基因atzA都高度保守。
对该菌及其他细菌中阿特拉津降解基因结构和排列的比较分析，有助于我们深入了解阿特拉津降解菌的生态学和进化。
