Reynolds R A, Segal S
Biochim Biophys Acta. 1976 Mar 19;426(3):513-25. doi: 10.1016/0005-2736(76)90395-3.
The uptaken of alpha-aminoisobutyric acid by slices of kidney cortex from newborn rats is enhanced by a preliminary incubation of the tissue in buffer at 37 degrees C. This effect is abolished by anaerobiosis, the presence of dinitrophenol or the removal of Na+ during the preliminary incubation. Cycloheximide (50 muM) and purimycin (1 mM) as well as alpha-aminoisobutyric acid, glycine and proline (5 mM) in the preincubation buffer also abolish the effect, while actinomycin D (0.8 muM) partially the phenomenon indicates that the enhanced uptake is due to an increased entry rate into the cells without a change in effux. There is no alteration in the apparent transport Km but an increase in the V for entry. The effect is dependent on tissue age being observed between birth and 22 days, after which there is a decrease in response to preliminary incubation with no effect seen in adult tissues.
新生大鼠肾皮质切片对α-氨基异丁酸的摄取，可通过将组织在37℃的缓冲液中预孵育而增强。厌氧、存在二硝基苯酚或在预孵育期间去除Na+可消除这种效应。预孵育缓冲液中的环己酰亚胺（50μM）、嘌呤霉素（1 mM）以及α-氨基异丁酸、甘氨酸和脯氨酸（5 mM）也可消除该效应，而放线菌素D（0.8μM）部分消除该效应。这表明摄取增强是由于进入细胞的速率增加而流出无变化。表观转运Km没有改变，但进入的V增加。该效应取决于组织年龄，在出生至22天之间可观察到，之后对预孵育的反应降低，在成年组织中未观察到效应。
