大鼠肝切片对蛋氨酸和脯氨酸的转运及某些激素的作用
Transport of methionine and proline by rat liver slices and the effect of certain hormones.
作者信息
Crawhall J C, Purkiss P
出版信息
Biochem J. 1973 Sep;136(1):15-24. doi: 10.1042/bj1360015.
Abstract
- Transport characteristics of l-methionine and l-proline in rat liver slices in vitro were studied. 2. Intracellular concentration gradients for methionine were obtained. 3. Methionine uptake was inhibited by iodoacetate, dinitrophenol, Na(+)-free media and also by glycine, lysine, cysteine and dithiothreitol but not by alpha-aminoisobutyrate. 4. The rate of methionine metabolism in the slice was slow. 5. Puromycin inhibited methionine incorporation into protein, but not methionine uptake. 6. Methionine inhibited the transport of alpha-aminoisobutyrate but not of cystine. 7. Efflux and exchange diffusion of methionine was studied. 8. Amino acid transport in rat liver slices was not affected by thyroidectomy. 9. Addition of insulin, glucagon, adrenaline or cortisol did not affect the transport of methionine. 10. Addition of 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate increased methionine transport after a 120min incubation period in some experiments. 11. Studies of l-proline transport were invalidated because of the rapid evolution of CO(2) from the substrate.
摘要
- 研究了大鼠肝切片中L-蛋氨酸和L-脯氨酸的转运特性。2. 获得了蛋氨酸的细胞内浓度梯度。3. 碘乙酸、二硝基苯酚、无钠培养基以及甘氨酸、赖氨酸、半胱氨酸和二硫苏糖醇可抑制蛋氨酸摄取，但α-氨基异丁酸无此作用。4. 切片中蛋氨酸的代谢速率缓慢。5. 嘌呤霉素抑制蛋氨酸掺入蛋白质，但不抑制蛋氨酸摄取。6. 蛋氨酸抑制α-氨基异丁酸的转运，但不抑制胱氨酸的转运。7. 研究了蛋氨酸的流出和交换扩散。8. 大鼠肝切片中的氨基酸转运不受甲状腺切除术的影响。9. 添加胰岛素、胰高血糖素、肾上腺素或皮质醇不影响蛋氨酸的转运。10. 在一些实验中，孵育120分钟后，添加6-N,2'-O-二丁酰腺苷3':5'-环一磷酸可增加蛋氨酸转运。11. 由于底物中二氧化碳的快速释放，L-脯氨酸转运的研究无效。
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